Patents Assigned to The Perkin-Elmer Corporation
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Publication number: 20020164591Abstract: The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O—P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases.Type: ApplicationFiled: February 27, 2001Publication date: November 7, 2002Applicant: The Perkin-Elmer CorporationInventors: John Brandis, Curtis Bloom, John H. Richards
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Publication number: 20020115067Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure 1Type: ApplicationFiled: October 11, 2001Publication date: August 22, 2002Applicant: The Perkin-Elmer CorporationInventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan D. Hauser
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Publication number: 20020094578Abstract: Generally, the present invention provides a device and method for accurately locating a multi-well plate at a plate-support location, e.g., a work surface, of an automated laboratory machine, such that one or more acting members of the machine, such as an array of pipette tips, optical sensors, or the like, can accurately address and operate on the individual wells. Interior and/or exterior surface regions of one or more wells of a multi-well plate are used as the primary plate features engaged by locating structure of the machine. In one particular embodiment one or more upwardly tapered projections extend from a plate-support surface of a plate-handling machine for mating engagement with the exterior surface regions of one or more wells. In another embodiment, the exterior surface regions along one or more well bottoms are engagingly received within bores, or other receiving structure, formed on the plate-support surface of a machine.Type: ApplicationFiled: December 6, 2001Publication date: July 18, 2002Applicant: The Perkin-Elmer CorporationInventors: Reid Burton Kowallis, David M. Cox
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Publication number: 20020081616Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.Type: ApplicationFiled: September 7, 2001Publication date: June 27, 2002Applicant: The Perkin-Elmer CorporationInventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
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Patent number: 6403812Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.Type: GrantFiled: May 30, 2000Date of Patent: June 11, 2002Assignee: The Perkin-Elmer CorporationInventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
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Patent number: 6395518Abstract: An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5′→3′ exonuclease activity of a polymerase and the 5′→3′ extension of by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification.Type: GrantFiled: May 19, 1997Date of Patent: May 28, 2002Assignee: The Perkin-Elmer CorporationInventor: Paul E. Mayrand
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Publication number: 20020045180Abstract: Propargylethoxyamino nucleosides are disclosed having the structure 1Type: ApplicationFiled: June 14, 2001Publication date: April 18, 2002Applicant: The Perkin-Elmer CorporationInventors: Shaheer H. Khan, Steven M. Menchen, Barnett B. Rosenblum
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Publication number: 20020034761Abstract: Dibenzorhodamine compounds having the structure 1Type: ApplicationFiled: October 2, 2001Publication date: March 21, 2002Applicant: The Perkin-Elmer CorporationInventors: Scott C. Benson, Joe Y.L. Lam, Steven Michael Menchen
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Patent number: 6358385Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: January 11, 1999Date of Patent: March 19, 2002Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
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Publication number: 20020029972Abstract: A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region and a detection zone, where the receiving, slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream the capillary outlets to the detection zone.Type: ApplicationFiled: September 4, 2001Publication date: March 14, 2002Applicant: The Perkin-Elmer CorporationInventors: Eric S. Nordman, John Shigeura, Albert L. Carrillo, David M. Demorest, Philip J. Wunderle
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Patent number: 6355709Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: October 15, 1997Date of Patent: March 12, 2002Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
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Publication number: 20020011444Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: ApplicationFiled: June 19, 2001Publication date: January 31, 2002Applicant: The Perkin-Elmer CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Patent number: 6333501Abstract: A system performs two types of spectral calibration. For the first type, the system collects data from monitoring an analytical separation of several spectrally-distinguishable molecular species and creates a data matrix from the collected data. Each element in the data matrix represents a signal intensity at a particular time and over a particular range of light wavelengths. The system then identifies regions in the data matrix that have spectral response characteristics of at least one of the molecular species, determines a set of pure component spectral responses from the identified regions, groups similar pure component spectral responses into clusters, determines a representative spectral response for each cluster, and correlates the representative spectral response for each cluster with one of the molecular species.Type: GrantFiled: January 27, 2000Date of Patent: December 25, 2001Assignee: Perkin-Elmer CorporationInventor: James N. Labrenz
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Patent number: 6329757Abstract: In a high frequency oscillator system a series oscillator circuit includes a load coil inductively coupled with a plasma generator. A capacitance is in series with the coil between the drain and source terminals of a transistor. A capacitive or inductive feedback responsive to the oscillation is connected to the gate terminal of the transistor. In another embodiment two sections of a series circuit are connected by the load coil, each section including a separate transistor. A first capacitance is connected between the coil and the first transistor drain, and a second capacitance is connected between the coil and the second transistor drain. A first capacitive feedback is connected between the second section and the first transistor gate, and a second capactive feedback is connected between the first section and the second transistor gate.Type: GrantFiled: December 31, 1996Date of Patent: December 11, 2001Assignee: The Perkin-Elmer CorporationInventors: Peter J. Morrisroe, Peter H. Gagne
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Patent number: 6326147Abstract: Systems, methods, graphical user interfaces, and articles of manufacture consistent with the present invention allow for biological assay preparation and automated biological macromolecule purification. Consistent with the present invention, a user may instruct the system to perform a series of assay preparation and purification procedures. A user may choose a series of procedures to be performed by a single automated workstation, one after the other, without human intervention. A user may specify, for example, a combination of lysis, archive, purification, and isolation protocols, including polymerase chain reaction (PCR). For each chosen procedure, a user may select a standard protocol or create a new protocol. Parameters for each of the protocols are cross-checked against parameters of other chosen protocols and system hardware for physical and rational limitations.Type: GrantFiled: May 13, 1999Date of Patent: December 4, 2001Assignee: The Perkin-Elmer CorporationInventors: Mark Floyd Oldham, Peter Carlton Honebein
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Patent number: 6326153Abstract: Dibenzorhodamine compounds having the structure are disclosed, including nitrogen and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C-14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.Type: GrantFiled: February 14, 2001Date of Patent: December 4, 2001Assignee: The Perkin-Elmer CorporationInventors: Scott C. Benson, Joe Y. L. Lam, Steven Michael Menchen
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Patent number: 6303775Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates.Type: GrantFiled: February 1, 2000Date of Patent: October 16, 2001Assignee: The Perkin-Elmer CorporationInventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan Hauser
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Patent number: 6297009Abstract: Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li+, Na+, or K+. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.Type: GrantFiled: January 9, 1998Date of Patent: October 2, 2001Assignee: Perkin-Elmer CorporationInventors: David M. Demorest, Stephen E. Moring, Claudia Chiesa
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Publication number: 20010019019Abstract: A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.Type: ApplicationFiled: March 13, 2001Publication date: September 6, 2001Applicant: The Perkin-Elmer CorporationInventors: Eric S. Nordman, John Shigeura, Albert L. Carrillo, David M. Demorest, Philip J. Wunderle
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Publication number: 20010011139Abstract: Dibenzorhodamine compounds having the structure 1Type: ApplicationFiled: February 14, 2001Publication date: August 2, 2001Applicant: The Perkin-Elmer CorporationInventors: Scott C. Benson, Joe Y.L. Lam, Steven Michael Menchen