Abstract: The present invention relates to pyrrolobenzoxazepines, pyrrolobenzthiazepines and related compounds having the ability to induce apoptosis, to pharmaceutical compositions comprising these compounds and to their use as anti-tumour agents.

Abstract: A semiconductor self-pulsating laser diode (1) comprises a wave guiding layer (2) sandwiched between lower and upper cladding layers (4, 5). A current blocking layer (8) defining a slot (10) through which pumping current is directed through the laser diode between upper and lower contact plates (5, 6) defines an active wave guiding region (15). The current blocking layer (8) is shaped by the formation of longitudinally extending recesses (12) for defining the active wave guiding region (15) such that a central pulse light generating region (17) is formed surrounded by an outer light propagating region (18). As the laser diode is continuously pumped, an effective step change in refractive index between the wave guiding layer (2) and the outer light propagating region (18) is formed, and the carrier density and refractive index profiles across the active wave guiding region (15) vary as each light pulse cycle progresses.

Abstract: Methods and reagents are provided for the tissue specific, cell specific and/or inducible expression of RNAi. The invention may be used to downregulate the expression of endogenous or exogenous RNAs targeted by the RNAi in cells, animals and/or plants.

Abstract: Biological assays using various constructions of biochips are disclosed to mirror in vivo situations. The biochip 50 comprises a microchannel 51 having a liquid outlet port 1, bubble release port 2 and a liquid outlet port 3 with an associated bubble release port 4. A multiplicity of tests can be performed often by coating the bore of the microchannel 50 which various adhesion mediating proteins or the use of chemoattractants. The assay assembly 60 comprises a syringe pump feeding the biochip 50. An inverted microscope 65, digital camera 66 and recorder 67 are provided. A sample liquid containing cells in suspension is injected slowly through the biochip and the effect of the assay recorded over a long period.

Abstract: Biological assays using various constructions of biochips are disclosed to mirror in vivo situations. The biochip 50 comprises a microchannel 51 having a liquid outlet port 1, bubble release port 2 and a liquid outlet port 3 with an associated bubble release port 4. A multiplicity of tests can be performed often by coating the bore of the microchannel 50 which various adhesion mediating proteins or the use of chemoattractants. The assay assembly 60 comprises a syringe pump feeding the biochip 50. An inverted microscope 65, digital camera 66 and recorder 67 are provided. A sample liquid containing cells in suspension is injected slowly through the biochip and the effect of the assay recorded over a long period.

Abstract: A dispensing assembly for liquid droplets that includes a dispenser connected to a liquid carrying pipe which in turn is connected to a source of pressurized liquid. The dispenser has an elongated body member having a main bore connected to the liquid carrying pipe. At the other end, the main bore has a valve seat that is connected to a nozzle having a nozzle bore terminating in a dispensing tip. An elongated valve boss of ferromagnetic material covered with a soft polymer is mounted in the main bore and has a cross-sectional area less than that of the main bore. A separate valve boss actuating coil assembly has upper and lower coils that are separate from the main body that can be unplugged from both coils and from the liquid carrying pipe. As such, the main body can be a disposable member.

Abstract: A dispensing assembly for liquid droplets that includes a dispenser connected to a liquid carrying pipe which in turn is connected to a source of pressurized liquid. The dispenser has an elongated body member having a main bore connected to the liquid carrying pipe. At the other end, the main bore has a valve seat that is connected to a nozzle having a nozzle bore terminating in a dispensing tip. A valve boss of ferromagnetic material covered with a soft polymer is mounted in the main bore and has a cross-sectional area less than that of the main bore. A separate valve boss actuating coil assembly has upper and lower coils that are separate from the main body that can be unplugged from both coils and from the liquid carrying pipe. As such, the main body can be a disposable member.

Abstract: Multicomponent vaccines are provided which aid in the prevention and treatment of staphylococcal infections and which include certain selected combinations of bacterial binding proteins or fragments thereof, or antibodies to those proteins or fragments. By careful selection of the proteins, fragments, or antibodies, a vaccine is provided that imparts protection against a broad spectrum of Staphylococcus bacterial strains and against proteins that are expressed at different stages of the logarithmic growth curve. In one embodiment of the invention, a composition is provided that includes at least a collagen binding protein or peptide (or an appropriate site directed mutated sequence thereof) such as CNA, or a protein or fragment with sufficiently high homology thereto, in combination with a fibrinogen binding protein, preferably Clumping factor A (“ClfA”) or Clumping factor B (“ClfB”), or a useful fragment thereof or a protein or fragment with sufficiently high homology thereto.

Abstract: A method and composition for the passive immunization of patients infected with or susceptible to infection from Staphylococcus bacteria such as S. aureus and S. epidermidis infection is provided that includes the selection or preparation of a donor plasma pool with high antibody titers to carefully selected Staphylococcus adhesins or MSCRAMMs, or fragments or components thereof, or sequences with substantial homology thereto. The donor plasma pool can be prepared by combining individual blood or blood component samples which have higher than normal titers of antibodies to one or more of the selected adhesins or other proteins that bind to extracellular matrix proteins, or by administering carefully selected proteins or peptides to a host to induce the expression of desired antibodies, and subsequently recovering the enhanced high titer serum or plasma pool from the treated host.

Abstract: Isolated proteins, designated SdrF, SdrG and SdrH, and their corresponding amino acid and nucleic acid sequences are provided which are useful in the prevention and treatment of infection caused by coagulase-negative staphylococcal bacteria such as S. epidermidis. The SdrF, SdrG and SdrH proteins are cell-wall associated proteins that specifically bind host proteins and which each have a highly conserved motif of which the consensus sequence is TYTFTDYVD (SEQ ID NO:16). The proteins, antigenic portions thereof and anti-SdrF, SdrG and SdrH antibodies are also useful for the identification and diagnosis of coagulase-negative staphylococcal infections. In particular, the proteins are advantageous because they may be used as vaccine components or antibodies thereof, and they may be administered to wounds or used to coat biomaterials to act as blocking agents to prevent or inhibit the binding of coagulase-negative staphylococci to wounds or biomaterials.

Abstract: Peptide derivatives useful as inhibitors of activity of thyrotropin-releasing hormone-degrading ectoenzyme (TRH-DE) disclosed.

Abstract: This invention relates to a process for purification of nanotube soot in a non-destructive and efficient method using a polymer having a coiling structure to extract nanotubes from their accompanying material without damage to their structure and with a high mass yield. Nanotube soot is added to a solvent which including a coiling polymer to form a solution. The solution is mixed with a nanotube composite suspension is formed with extraneous solid material such as amorphous carbon settling at the bottom of the solution. The nanotube composite suspension is decanted from the settled solid.

Abstract: A device (1) for acquiring cell samples by aspiration into an evacuated container (3) comprising: a front hollow needle portion (7) to be inserted into the body from which a sample is to be taken; a rear hollow needle portion (5) for communication with the evacuated container; a conduit (11) connecting the front needle portion (7) to the rear needle portion (5); and a normally closed pinch valve mechanism (6) to open and close the conduit; The valve mechanism (6) is manually operable by one hand and controls the application of negative pressure in the front needle portion (7). The conduit may comprise a flexible tube and the valve mechanism (6) may comprise releasable means (8) to pinch the flexible tube in a pressure-tight manner. The valve mechanism (6) may comprise a spring-biased push-button (8) operable by one digit of a user's hand.

Abstract: A structuring device (SD) for processing a surface of a substrate (SB), comprising a substrate chamber (VC) for mounting the substrate (SB) and a reaction chamber (GC) enabling a gas reaction at a given operating pressure. The reaction chamber (GC) has at least one gas inlet (GL) for a reaction gas and at least one injection outlet (JL) leading into the substrate chamber, while the substrate chamber (VC) is provided with a pumping system (PP) for maintaining a vacuum within the substrate chamber at a pressure not above the operating pressure of the gas reaction in the reaction chamber (GC). The injection outlet (JL) is provided with at least one injection pipe ending into an injection opening of given width, the injection pipe having a length not smaller than the width of the injection opening, the injection pipe forming the gas particles originating from the gas reaction into a gas jet streaming out of the injection opening.

Abstract: The present invention is drawn to a method for the quantitative determination of human acute phase serum amyloid A protein (A-SAA) comprising contacting a sample of a biological fluid with antibody specific for A-SAA, the sample being reacted with an organic solvent prior to or simultaneous with antibody contact. The organic solvent is suitably a C1 or C4 alcohol and the antibody can be used on the solid phase and as a component of the detection system of an enzyme linked immunosorbant assay. The method provides a sensitive and reliable measure of A-SAA and inflammatory status which can be used for the diagnosis and clinical management of both acute and chronic inflammatory conditions.

Abstract: The isolation of the S. aureus fibrinogen binding protein gene is described and a minimal fibrinogen binding protein is identified. The protein finds use as a vaccine or a pharmaceutical composition for application to prevent infection, promotion of wound healing, blocking adherence to indwelling medical devices, or diagnosis of infection.

Abstract: The isolation of the S. aureus fibrinogen binding protein gene is described and a minimal fibrinogen binding protein is identified. The protein finds use as a vaccine or a pharmaceutical composition for application to prevent infection, promotion of wound healing, blocking adherence to indwelling medical devices, or diagnosis of infection.

Abstract: The present invention relates to a live attenuated strain of the bacterium Streptococcus equi, a pathogen causing strangles in horses. The invention also relates to a vaccine against strangles, methods for the preparation of such a vaccine and to the use of the strain for the preparation of such a vaccine.

Abstract: A process is provided for modifying the magnetic properties of an intermetallic compound comprising at least iron, or a combination of iron with at least one transition metal, and at least one rare earth element. The process comprises heating the intermetallic compound in a reaction gas containing at least one element of groups IIIA, IVA or VIA of the Periodic Table in the gaseous phase to interstitially incorporate the element or elements of these groups into the crystal lattice of the intermetallic compound. Novel magnetic materials showing easy uniaxial anisotropy, increased spontaneous magnetization and Curie temperatures are produced by the process.

Abstract: A method and apparatus for thermal analysis of a solid or liquid sample which evolves or absorbs gas on heating or cooling, wherein the sample is heated or cooled in an enclosed space, the temperature of the sample and the pressure of the gas are continuously monitored by temperature and pressure sensors respectively, and measurements of sample temperature and gas pressure are input to a data processor which also controls the temperature change of the sample. The value of gas pressure as a function of sample temperature and the rate of change of this value with respect to sample temperature are calculated; these functions are characteristic of the sample or of a component of the sample under analysis.