Patents Assigned to The Public Health Institute of the City of New York, Inc.
  • Wavelength-shifting probes and primers and their use in assays and kits
    Patent number: 6037130
    Abstract: Hairpin-forming oligonucleotide probes and primers triple-labeled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes an increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided.
    Type: Grant
    Filed: July 28, 1998
    Date of Patent: March 14, 2000
    Assignee: The Public Health Institute of the City of New York, Inc.
    Inventors: Sanjay Tyagi, Fred R. Kramer, Salvatore A. E. Marras