Abstract: There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a protein that is an RNA-directed RNA ligase. Preferred assays include generation of an exponentially amplified signal. Most preferred assays include T4 DNA ligase as the RNA-directed RNA ligase. Also provided are kits for performing assays according to this invention.
Type:
Grant
Filed:
December 12, 1994
Date of Patent:
September 15, 1998
Assignee:
The Public Health Research Institute of New York, Inc.