Abstract: The invention provides an attenuated poliovirus which does not have a base pair mismatch in stem (a) or (b) of domain V of the 5? non-coding region of its genome, wherein at least seven of the base pairs in stems (a) and (b) are U-A or A-U base pairs.
Abstract: An assay is provided for detecting the activity of a reporter kinase comprising (i) adding said reporter kinase to an assay mixture wherein said reporter kinase is contacted with bioluminescent reagent no more than 5 minutes after being contacted with ADP, and wherein, prior to contacting the reporter kinase with ADP, the assay mixture is substantially free from kinase other than reporter kinase; and (ii) detecting light output from the assay mixture. Methods for detecting the presence of an analyte in a sample and methods for validating a treatment process using the above assay are also provided. Further provided are devices for conducting these assays and methods.
Type:
Application
Filed:
August 2, 2013
Publication date:
January 30, 2014
Applicant:
The Secretary of State for Health
Inventors:
MARK J. SUTTON, TORYN POOLMAN, RICHARD J. HESP
Abstract: The invention provides an attenuated polio virus having a 5? non-coding region consisting of the 5? non-coding region of Sabin 3, modified so that it does not have a base pair mismatch in stem (a) or (b) of domain V, wherein seven or eight of the base pairs in stems (a) and (b) are U-A or A-U base pairs; and a capsid protein from the Sabin 1, Mahoney, MEF or Saukett strain.
Abstract: The invention provides an attenuated poliovirus which does not have a base pair mismatch in stem (a) or (b) of domain V of the 5? non-coding region of its genome, wherein at least seven of the base pairs in stems (a) and (b) are U-A or A-U base pairs.
Abstract: An assay is provided for detecting the activity of a reporter kinase comprising (i) adding said reporter kinase to an assay mixture wherein said reporter kinase is contacted with bioluminescent reagent no more than minutes after being contacted with ADP, and wherein, prior to contacting the reporter kinase with ADP, the assay mixture is substantially free from kinase other than reporter kinase; and (ii) detecting light output from the assay mixture. Methods for detecting the presence of an analyte in a sample and methods for validating a treatment process using the above assay are also provided. Further provided are devices for conducting these assays and methods.
Type:
Grant
Filed:
January 7, 2010
Date of Patent:
August 20, 2013
Assignee:
The Secretary of State for Health
Inventors:
Mark J. Sutton, Toryn Poolman, Richard J. Hesp
Abstract: A biocompatible moldable curable polymer/monomer system capable of curing at ambient temperatures to provide an elastomer product while producing a lower exotherm than previously used systems. The system includes an alkyl acrylate or methacrylate homopolymer or copolymer; a monomer mixture of higher alkylacrylate or methacrylate combined with one or more of acrylic acid, methacrylic acid and their lower alkyl esters; and a plasticizer and optionally cross-linking agents, anti-tack agents, and activators or photoinitiators. The moldable systems is a dough used for making earmoulds and earpieces or in fabrication of prostheses.
Type:
Grant
Filed:
July 19, 1993
Date of Patent:
March 28, 1995
Assignee:
The Secretary of State for Health in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland