Abstract: Disclosed are fluorescent protein sensors for measuring the pH of a sample, nucleic acids encoding them, and methods of use. The preferred fluorescent protein sensors are variants of the green fluorescent protein (GFP) from Aequorea victoria. Also disclosed are compositions and methods for measuring the pH of a specific region of a cell, such as the mitochondrial matrix or the Golgi lumen.
Type:
Grant
Filed:
October 13, 1998
Date of Patent:
November 21, 2000
Assignees:
The Regents of the University of California, The State of Oregon acting by and through the State of Board of Higher Education on behalf of the Unviersity of Oregon
Inventors:
Roger Y. Tsien, Juan Llopis, Rebekka M. Wachter, S. James Remington