Patents Assigned to The United States of America as Represented by the Secretary of Defense on behalf of the Armed Forces Institute of Pathology
  • Pressure-assisted molecular recovery (PAMR) of biomolecules, pressure-assisted antigen retrieval (PAAR), and pressure-assisted tissue histology (PATH)
    Patent number: 8481283
    Abstract: A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: July 9, 2013
    Assignees: The United States of America as Represented by the Department of Veterans Affairs, The United States of America as Represented by the Secretary of Defense on behalf of the Armed Forces Institute of Pathology, American Registry of Pathology
    Inventors: Timothy J. O'Leary, Jeffrey T. Mason, Carol B. Fowler, Robert E. Cunningham
  • Pressure-assisted molecular recovery (PAMR) of biomolecules, pressure-assisted antigen retrieval (PAAR), and pressure-assisted tissue histology (PATH)
    Patent number: 8288122
    Abstract: A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: October 16, 2012
    Assignees: The United States of America as Represented by the Department of Veterans Affairs, The United States of America as Represented by the Secretary of Defense on behalf of the Armed Forces Institute of Pathology, American Registry of Pathology
    Inventors: Timothy J. O'Leary, Jeffrey T. Mason, Carol B. Fowler, Robert E. Cunningham