Abstract: Eucaryotic cells like mammal and plant cells transfected with genes, called retrons, which code for stable single-stranded hybrid molecules (msDNA) containing RNA and DNA portions are disclosed. The retrons producing said linked RNA and DNA portions of the msDNAs also contain a gene encoding a reverse transcriptase (RT), which is necessary for the synthesis of the msDNAs. The msDNAs may contain foreign nucleic acid fragments which may be antisense fragments. These msDNA antisense vectors are useful for targeting genes of target proteins.
Type:
Grant
Filed:
July 25, 1995
Date of Patent:
December 15, 1998
Assignee:
The University of Medecine and Dentistry of New Jersey
Abstract: A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.
Type:
Grant
Filed:
May 4, 1995
Date of Patent:
February 3, 1998
Assignee:
The University of Medecine and Dentistry of New Jersey