Abstract: This invention relates to the in vitro production of cyclic peptides using cyanobacterial enzymes, such as patellamide biosynthesis enzymes. Linear peptide substrates are cyclized using an isolated cyanbacterial macrocyclase, such as PatG from Prochloron spp. Before cyclisation, residues in the linear peptide substrates may be heterocyclised using isolated cyanbacterial heterocyclases, such as PatD or TruD heterocyclase. Methods of the invention may be useful, for example, for the production of cyclic peptidyl molecules, including cyclotides, such as katalas, and cyanobactins, such as patellamides and telomestatins, for example for use in the development of therapeutics.
Type:
Application
Filed:
June 28, 2013
Publication date:
November 12, 2015
Applicants:
The University of the University of Aberdeen, The University Court of the University of St. Andrews
Inventors:
Wael Houssen Ibrahim, Marcel Jaspars, Margaret Smith, James Naismith, Jesko Koehnke, Andrew Bent, Nicholas Westwood