Abstract: Described herein is the finding that hyaluronan antagonists that inhibit hyaluronan signaling are capable of inhibiting airway inflammation and airway hyperresponsiveness (AHR). The present disclosure provides a method of preventing or reducing AHR in a subject suffering from or at risk for AHR by administering a hyaluronan antagonist. Also provided is a method of treating an airway disease or disorder in a subject by administering a hyaluronan antagonist. Hyaluronan antagonists include, for example, heparosan and hyaluronan oligosaccharides (oHAs). In some embodiments, the hyaluronan antagonist is administered locally to the airway, such as with an inhaler or nebulizer.
Type:
Application
Filed:
March 8, 2013
Publication date:
April 16, 2015
Applicants:
The United States of America, as represented by the Secretary, Dept. of Health and Human Services, Duke University, MUSC Foundation for Research Development, The Universtiy of North Carolina at Chapel Hill
Inventors:
Stavros Garantziotis, John W. Hollingsworth, Bryan P. Toole, Jian Liu
Abstract: An electrode and method of preparing an electrode by electropolymerizing a film on the conductive working surface of an electrode. The electrode is modified by reductive electropolymerization of a thin film of poly[Ru(vbpy)32+] or poly[Ru(vbpy)32+/vba] (vbpy=4-vinyl-4′methyl-2,2′-bipyridine and vba=p-vinylbenzoic acid) and the electrode is used for the electrochemical detection of aqueous GMP, poly[G], and surface immobilized single-stranded DNA probes. The film is formed from a co-polymer of a mediator such as Ru(vbpy)32+ and a functionalized moiety having a carboxylate group such as p-vinylbenzoic acid. A DNA probe is attached covalently to the carboxylate group via a carbodiimide reaction followed by amidation of an amino-linked single-stranded DNA.
Type:
Grant
Filed:
March 12, 1999
Date of Patent:
January 30, 2001
Assignee:
The Universtiy of North Carolina at Chapel Hill