Abstract: Galactose oxidase enzyme can effectively be used for the determination of a number of different substances if activated and stabilized in accordance with the present invention. Activation is accomplished with a redox agent such as ferricyanide. Stabilization of the activated galactose oxidase is accomplished by addition of cupric ion. Upon stabilization, activated, immobilized galactose oxidase enzymes may be stored and reused for at least 25 days.