Abstract: Multi-layer devices suitable for thermal cycling processes. The devices are particularly suitable for performing polymerase chain reactions (PCR). One embodiment includes a first conducting layer, a second conducting layer adjacent to the first layer, and a third conducting layer adjacent to the second layer opposite the first layer. Insulating layers are positioned between said three conducting layers. Continuous channels are formed within the layers. The channels can be formed in either the conducting layer or the insulating layers, or both. Other embodiments include two conducting layers. At least one integral or separate temperature source may be provided to maintain the conducting layers at various desired temperatures.

Abstract: A multi-layer device suitable for thermal cycling is set forth. The device is particularly suitable for performing a polymerase chain reaction (PCR). One embodiment includes a first layer (18), a second layer (20) adjacent to the first layer, and a third layer (22) adjacent to the second layer opposite the first layer. A continuous channel (54) is formed within the first layer (18), the second layer (20), and the third layer (22). The continuous channel (54) has a plurality of cycle segments, each of the cycle segments comprises a first portion (58) disposed within the first layer (18), a second portion (62) disposed within the third layer (22), and a third portion (66) disposed within the second layer (20). An integral or separate temperature source or sources (14), (16) may be provided to maintain the first layer (18) at a first temperature and the third layer (22) at a second temperature.

Abstract: A multi-layer device suitable for thermal cycling is set forth. The device is particularly suitable for performing a polymerase chain reaction (PCR). One embodiment includes a first layer (18), a second layer (20) adjacent to the first layer, and a third layer (22) adjacent to the second layer opposite the first layer. A continuous channel (54) is formed within the first layer (18), the second layer (20), and the third layer (22). The continuous channel (54) has a plurality of cycle segments, each of the cycle segments comprises a first portion (58) disposed within the first layer (18), a second portion (62) disposed within the third layer (22), and a third portion (66) disposed within the second layer (20). An integral or separate temperature source or sources (14), (16) may be provided to maintain the first layer (18) at a first temperature and the third layer (22) at a second temperature.