Patents Assigned to Thermo Fisher Scientific Baltics, UAB
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Publication number: 20240102003Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.Type: ApplicationFiled: June 30, 2023Publication date: March 28, 2024Applicants: Thermo Fisher Scientific Baltics UAB, Life Technologies CorporationInventors: Arvydas Lubys, Inga Cikotiene, Zana Kapustina, Arturas Berezniakovas, Justina Sulgaite, Simona Zeimyte, Mark Andersen, Michael Allen, Sihong Chen
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Publication number: 20230357732Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. The thermophilic family B DNA polymerases provided herein also comprise an N-terminal domain comprising a uracil-binding pocket that has been modified to reduce template uracil binding. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence nonspecific dsDNA binding domain.Type: ApplicationFiled: April 3, 2023Publication date: November 9, 2023Applicant: Thermo Fisher Scientific Baltics UABInventors: Rasa Sukackaite, Skaiste Valinskyte
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Patent number: 11739316Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligonucleotides can be used in a variety of nucleic acid sequencing methods.Type: GrantFiled: June 22, 2020Date of Patent: August 29, 2023Assignees: Thermo Fisher Scientific Baltics UAB, Life Technologies CorporationInventors: Arvydas Lubys, Inga Cikotiene, Zana Kapustina, Arturas Berezniakovas, Justina Medziune, Simona Zeimyte, Mark Andersen, Michael Allen, Sihong Chen
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Publication number: 20230174957Abstract: This application relates to mutant polymerases. This application discloses mutant phage-type RNA polymerases, such as a mutant T7, SP6, and T3 RNA polymerase, may use 2?-modified nucleoside triphosphates or deoxynucleotide triphosphates as substrates. Methods for producing nucleic acid molecules using these mutant polymerases are also disclosed.Type: ApplicationFiled: May 11, 2021Publication date: June 8, 2023Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Lubys, Zana Kapustina, Aiste Jaspone
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Patent number: 11618891Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. The thermophilic family B DNA polymerases provided herein also comprise an N-terminal domain comprising a uracil-binding pocket that has been modified to reduce template uracil binding. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: June 25, 2018Date of Patent: April 4, 2023Assignee: Thermo Fisher Scientific Baltics UABInventors: Rasa Sukackaite, Skaiste Valinskyte
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Patent number: 11560553Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: April 8, 2020Date of Patent: January 24, 2023Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Publication number: 20220275447Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: ApplicationFiled: October 11, 2021Publication date: September 1, 2022Applicant: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Patent number: 11286345Abstract: Disclosed herein are poly(hydroxylalkyleneimine disulfide)polymers, which have both desirable transfection properties and reduced toxicity.Type: GrantFiled: March 10, 2017Date of Patent: March 29, 2022Assignee: Thermo Fisher Scientific Baltics UABInventors: Sarunas Zigmantas, Lolita Zaliauskiene, Ricardas Makuska, Alma Bockuviene, Ausvydas Vareikis
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Patent number: 11268084Abstract: Glycerol-free enzyme formulations are described. In some embodiments, a glycerol-free enzyme formulation is stabilized by high salt concentration. The glycerol free enzyme formulation may comprise a reverse transcriptase enzyme.Type: GrantFiled: August 2, 2018Date of Patent: March 8, 2022Assignee: Thermo Fisher Scientific Baltics, UABInventors: Juozas Siurkus, Darius Kavaliauskas, Daumantas Matulis, Lina Baranauskiene
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Patent number: 11136576Abstract: A composition and method for controlled in vitro fragmentation of nucleic acids. A transposase forms catalytically active complexes with a modified transposon end that contains within its end sequence degenerate, apurinic/apyrimidinic sites, nicks, or nucleotide gaps, to fragment or shear a target nucleic acid sample in a controlled process. This method yields desired average nucleic acid fragment sizes. The inventive composition and method may be applied for generation of DNA fragments containing shortened transposon end sequences to facilitate subsequent reactions, for production of asymmetrically tailed DNA fragments, etc.Type: GrantFiled: November 16, 2016Date of Patent: October 5, 2021Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Mindaugas Ukanis, Arvydas Lubys, Romas Tamosevicius, Ervinas Gaidamauskas
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Publication number: 20200308643Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: ApplicationFiled: March 11, 2020Publication date: October 1, 2020Applicant: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Patent number: 10731152Abstract: A composition and method for controlled in vitro fragmentation of nucleic acids. A transposase forms catalytically active complexes with a modified transposon end that contains within its end sequence degenerate, apurinic/apyrimidinic sites, nicks, or nucleotide gaps, to fragment or shear a target nucleic acid sample in a controlled process. This method yields desired average nucleic acid fragment sizes. The inventive composition and method may be applied for generation of DNA fragments containing shortened transposon end sequences to facilitate subsequent reactions, for production of asymmetrically tailed DNA fragments, etc.Type: GrantFiled: November 17, 2015Date of Patent: August 4, 2020Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Mindaugas Ukanis, Arvydas Lubys, Romas Tamo{hacek over (s)}evicius, Ervinas Gaidamauskas
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Patent number: 10626383Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: January 13, 2017Date of Patent: April 21, 2020Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Patent number: 10626461Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: GrantFiled: May 25, 2018Date of Patent: April 21, 2020Assignee: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Patent number: 10590399Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: GrantFiled: September 29, 2016Date of Patent: March 17, 2020Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubelevic̆iūtē, Renata Rims̆eliēnē, Sigitas Burinskas
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Patent number: 10435675Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: GrantFiled: February 1, 2017Date of Patent: October 8, 2019Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Patent number: 10358670Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: April 20, 2018Date of Patent: July 23, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: D861915Type: GrantFiled: September 13, 2017Date of Patent: October 1, 2019Assignees: THERMO FISHER SCIENTIFIC BALTICS UAB, THERMO FISHER ISRAEL LTDInventors: Linas Zakrys, Paulius Mielinis, Ronen Benarieh
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Patent number: D905273Type: GrantFiled: July 23, 2019Date of Patent: December 15, 2020Assignees: THERMO FISHER SCIENTIFIC BALTICS UAB, THERMO FISHER ISRAEL LTDInventors: Linas Zakrys, Paulius Mielinis, Ronen Benarieh
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Patent number: D919835Type: GrantFiled: February 10, 2020Date of Patent: May 18, 2021Assignees: THERMO FISHER SCIENTIFIC BALTICS UAB, THERMO FISHER ISRAEL LTDInventors: Justas Morkunas, Ronen Ben Arieh, Shahar Schlezinger