Patents Assigned to Thermogen, Inc.
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Patent number: 7659110Abstract: A processing block 2 is composed of a base 5, where an upper substrate 6 formed with a metal material M and a lower substrate 7 formed with the metal material M or a ceramic material E are adhered, and cells C . . . supported by this base 5; and the cells C . . . are secured to the upper substrate 6 and/or the lower substrate 7 at least via cell positioners 6s . . . established in the upper substrate 6 for positioning the cells C . . . , respectively. At the same time, at least the thickness Ld of regions Xc . . . situated under the cells C . . . in the lower substrate 7 is selected to be 1.0 [mm] or thinner, and, a thermo-module(s) comes into contact with the lower surface of the base 5.Type: GrantFiled: June 14, 2005Date of Patent: February 9, 2010Assignee: Thermogen Inc.Inventors: Seiichi Kudoh, Ryoji Kobayashi
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Publication number: 20040014161Abstract: Provided herein is a method for the quantitative screening of hydrolase for desired substrate activity using pH indicators which are sensitive to the release of protons from a chemical reaction in a reaction mixture. The method comprises selecting buffer and indicator conditions such that both have the same affinity for protons such that the relative amount of buffer protonated is proportional to the amount of indicator protonated as the pH of the reaction mixture shifts. A reaction mixture is then prepared comprising a buffer, indicator, hydrolase to be tested, and desired substrate to be tested, allowing the hydrolase to react with the substrate. The reaction is monitored by detection of change in color of the reaction mixture, determined by the affect of the reaction on the indicator.Type: ApplicationFiled: June 30, 2003Publication date: January 22, 2004Applicant: ThermoGen, Inc.Inventors: Lana Elizabeth Janes, Christina A. Lowendahl, Romas J. Kazlauskas
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Publication number: 20030059816Abstract: This invention describes methods for the identification of enzymes from the enolase superfamily with racemase activity for industrial applications. The methods comprise obtaining a micro-organism which requires the enzyme with the acquired racemase activity to grow in a selective medium. The micro-organism is transformed with a gene encoding the enzyme, grown in the selective medium supplemented with a substrate of the racemase, and one or more micro-organism(s) that is able to grow in the supplemented selective medium is then isolated, thereby identifying the enzyme with the acquired activity. The methods further comprise genetic alterations of the genes that encode enzymes resulting in the acquired racemase.Type: ApplicationFiled: August 2, 2002Publication date: March 27, 2003Applicant: Thermogen, Inc.Inventors: Ian Fotheringham, Sylvain Royer
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Publication number: 20020177180Abstract: Provided herein is a method for the quantitative screening of hydrolase for desired substrate activity using pH indicators which are sensitive to the release of protons from a chemical reaction in a reaction mixture. The method comprises selecting buffer and indicator conditions such that both have the same affinity for protons such that the relative amount of buffer protonated is proportional to the amount of indicator protonated as the pH of the reaction mixture shifts. A reaction mixture is then prepared comprising a buffer, indicator, hydrolase to be tested, and desired substrate to be tested, allowing the hydrolase to react with the substrate. The reaction is monitored by detection of change in color of the reaction mixture, determined by the affect of the reaction on the indicator.Type: ApplicationFiled: January 24, 2002Publication date: November 28, 2002Applicant: ThermoGen, Inc.Inventors: Lana Elizabeth Janes, Christina A. Lowendahl, Romas J. Kazlauskas
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Patent number: 6350591Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kantr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu−Kmr transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.Type: GrantFiled: February 16, 1999Date of Patent: February 26, 2002Assignee: Thermogen, Inc.Inventors: J. Mark Weber, David C. Demirjian, Malcolm J. Casadaban, Veronika Vonstein, Nikos C. Pagratis
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Patent number: 6344327Abstract: The present invention relates to a system for identifying, isolating and utilizing promoter elements useful for expression of nucleotide sequences and the proteins encoded thereby in a thermophile. In one embodiment, a recombinant DNA molecule is provided, and comprises a reporter sequence, a putative thermophile promoter, a selectable marker sequence, and a 3′ and a 5′ DNA targeting sequence that are together capable of causing integration of at least a portion of said DNA molecule into the genome of a thermophile. Further, within the recombinant DNA, the reporter sequence is under the transcriptional control of a promoter which functions in a thermophile to form a promoter/reporter cassette, the promote/reporter cassette is flanked by said 3′ and said 5′ DNA targeting sequences, and the promoter/reporter cassette is positioned in the opposite orientation of the DNA targeting sequences.Type: GrantFiled: April 12, 2000Date of Patent: February 5, 2002Assignee: Thermogen, Inc.Inventors: Mikhail Peredultchuk, Veronica Vonstein, David Demirjian
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Patent number: 6294358Abstract: The present invention relates to a system for identifying, isolating and utilizing promoter elements useful for expression of nucleotide sequences and the proteins encoded thereby in a thermophile. In one embodiment, a recombinant DNA molecule is provided, and comprises a reporter sequence, a putative thermophile promoter, a selectable marker sequence, and a 3′ and a 5′ DNA targeting sequence that are together capable of causing integration of at least a portion of said DNA molecule into the genome of a thermophile. Further, within the recombinant DNA, the reporter sequence is under the transcriptional control of a promoter which functions in a thermophile to form a promoter/reporter cassette, the promote/reporter cassette is flanked by said 3′ and said 5′ DNA targeting sequences, and the promoter/reporter cassette is positioned in the opposite orientation of the DNA targeting sequences.Type: GrantFiled: September 7, 1999Date of Patent: September 25, 2001Assignee: Thermogen, Inc.Inventors: Mikhail Peredultchuk, Veronica Vonstein, David C. Demirjian
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Patent number: 6218163Abstract: The instant invention encompasses isolated stable esterase enzymes characterized by the ability to remain stable at certain temperatures, substrate specificities, and activity profile.Type: GrantFiled: August 8, 1996Date of Patent: April 17, 2001Assignee: Thermogen, Inc.Inventors: Larry Allen, John Aikens, David Demirjian, Veronika Vonstein, Michael Fonstein, Malcolm Casadaban
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Patent number: 6218167Abstract: The instant invention encompasses isolated stable esterase enzymes characterized by the ability to remain stable at certain temperatures, substrate specificities, and activity profile; the expression vectors which can express, nucleic acids which encode for, and corresponding protein amino acid sequence of such proteins.Type: GrantFiled: April 10, 1998Date of Patent: April 17, 2001Assignee: ThermoGen, Inc.Inventors: Larry Allen, John Aikens, David DeMirjian, Veronika Vonstein, Michael Fonstein, Malcolm Casadaban
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Patent number: 5969121Abstract: The instant invention encompasses isolated stable esterase enzymes characterized by the ability to remain stable at certain temperatures, substrate specificities, and activity profile.Type: GrantFiled: January 10, 1997Date of Patent: October 19, 1999Assignee: Thermogen, Inc.Inventors: Larry Allen, John Aikens, Michael Fonstein, Veronika Vonstein, David Demirjian, Malcolm Casadaban
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Patent number: 5872238Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan.sup.tr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu.sup.- Km.sup.r transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.Type: GrantFiled: August 18, 1997Date of Patent: February 16, 1999Assignee: Thermogen, Inc.Inventors: J. Mark Weber, David C. Demirjian, Malcolm J. Casadaban, Veronika Vonstein, Nikos C. Pagratis
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Patent number: 5786174Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan.sup.tr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu.sup.- Km.sup.r transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.Type: GrantFiled: January 28, 1997Date of Patent: July 28, 1998Assignee: Thermogen, Inc.Inventors: J. Mark Weber, David C. Demirjian, Malcolm J. Casadaban, Nikos C. Pagratis, Veronika Vonstein