Patents Assigned to Third Wave Technologies
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Patent number: 9133503Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.Type: GrantFiled: May 24, 2012Date of Patent: September 15, 2015Assignee: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Victor Lyamichev
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Patent number: 8911973Abstract: The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3? hairpin structures, 5? hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5? or 3? ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.Type: GrantFiled: May 21, 2013Date of Patent: December 16, 2014Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Andrew A. Lukowiak, Patrick Peterson
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Patent number: 8765418Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: February 15, 2013Date of Patent: July 1, 2014Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Publication number: 20140004514Abstract: The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3? hairpin structures, 5? hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5? or 3? ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.Type: ApplicationFiled: May 21, 2013Publication date: January 2, 2014Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Jeff G. Hall, Andrew A. Lukowiak, Patrick Peterson
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Patent number: 8609336Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.Type: GrantFiled: February 7, 2012Date of Patent: December 17, 2013Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Hatim T Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn Olson-Munoz, LuAnne Chehak, Sarah M. Olson
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Patent number: 8552173Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.Type: GrantFiled: August 22, 2011Date of Patent: October 8, 2013Assignee: Third Wave Technologies, Inc.Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
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Patent number: 8445238Abstract: The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3? hairpin structures, 5? hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5? or 3? ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.Type: GrantFiled: October 3, 2007Date of Patent: May 21, 2013Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Andrew A. Lukowiak, Patrick Peterson
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Patent number: 8389245Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: September 19, 2007Date of Patent: March 5, 2013Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8354232Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.Type: GrantFiled: July 20, 2010Date of Patent: January 15, 2013Assignee: Third Wave Technologies, Inc.Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor I. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
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Patent number: 8288093Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.Type: GrantFiled: April 8, 2008Date of Patent: October 16, 2012Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
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Publication number: 20120231461Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.Type: ApplicationFiled: May 24, 2012Publication date: September 13, 2012Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Hatim T. Allawi, Victor Lyamichev
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Patent number: 8206904Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.Type: GrantFiled: June 1, 2007Date of Patent: June 26, 2012Assignee: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Victor Lyamichev
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Publication number: 20120149018Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.Type: ApplicationFiled: February 7, 2012Publication date: June 14, 2012Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
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Patent number: 8190371Abstract: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.Type: GrantFiled: September 8, 2008Date of Patent: May 29, 2012Assignee: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Victor Lyamichev, Rex Piper
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Patent number: 8182991Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostable organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: October 6, 2000Date of Patent: May 22, 2012Assignee: Third Wave Technologies, Inc.Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamicheva
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Publication number: 20120010395Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.Type: ApplicationFiled: August 22, 2011Publication date: January 12, 2012Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Zbigniew Skrzypczynski, Sarah R. Wayland
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Patent number: 8080643Abstract: The present invention provides methods, kits, and compositions related to human papillomavirus (HPV) primers, which may be used, for example, in nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays.Type: GrantFiled: September 5, 2007Date of Patent: December 20, 2011Assignee: Third Wave Technologies, Inc.Inventors: Joseph J. King, Angela Hudson
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Patent number: 8071750Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.Type: GrantFiled: December 30, 2008Date of Patent: December 6, 2011Assignee: Third Wave Technologies, Inc.Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
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Patent number: 8063184Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: October 5, 2010Date of Patent: November 22, 2011Assignee: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, Jr., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Patent number: 8026050Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.Type: GrantFiled: September 13, 2000Date of Patent: September 27, 2011Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev