Patents Assigned to Third Wave Technologies, Inc.
  • Patent number: 9133503
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: September 15, 2015
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Allawi, Victor Lyamichev
  • Patent number: 8911973
    Abstract: The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3? hairpin structures, 5? hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5? or 3? ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.
    Type: Grant
    Filed: May 21, 2013
    Date of Patent: December 16, 2014
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Andrew A. Lukowiak, Patrick Peterson
  • Patent number: 8765418
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Grant
    Filed: February 15, 2013
    Date of Patent: July 1, 2014
    Assignee: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Patent number: 8609336
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: December 17, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Hatim T Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • Patent number: 8552173
    Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.
    Type: Grant
    Filed: August 22, 2011
    Date of Patent: October 8, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
  • Patent number: 8445238
    Abstract: The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3? hairpin structures, 5? hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5? or 3? ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.
    Type: Grant
    Filed: October 3, 2007
    Date of Patent: May 21, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Andrew A. Lukowiak, Patrick Peterson
  • Patent number: 8389245
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Grant
    Filed: September 19, 2007
    Date of Patent: March 5, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Patent number: 8354232
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: January 15, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor I. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Patent number: 8288093
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: October 16, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
  • Patent number: 8206904
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: June 26, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev
  • Patent number: 8190371
    Abstract: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.
    Type: Grant
    Filed: September 8, 2008
    Date of Patent: May 29, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev, Rex Piper
  • Patent number: 8182991
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostable organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: May 22, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamicheva
  • Patent number: 8080643
    Abstract: The present invention provides methods, kits, and compositions related to human papillomavirus (HPV) primers, which may be used, for example, in nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays.
    Type: Grant
    Filed: September 5, 2007
    Date of Patent: December 20, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Joseph J. King, Angela Hudson
  • Patent number: 8071750
    Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: December 6, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
  • Patent number: 8063184
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: October 5, 2010
    Date of Patent: November 22, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, Jr., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Patent number: 8026050
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: September 13, 2000
    Date of Patent: September 27, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
  • Patent number: 8003771
    Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.
    Type: Grant
    Filed: March 1, 2010
    Date of Patent: August 23, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Zbigniew Skrzypczynski, Sarah R Wayland
  • Patent number: 7939257
    Abstract: The present invention relates to methods and compositions for predicting the risk of obesity. In particular, the present invention provides methods and compositions for determining a subject's risk of obesity based on the presence of polymorphisms in the growth hormone secretagogue receptor (GHSR).
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: May 10, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Anne E. Kwitek, Andrea Baessler
  • Patent number: 7935800
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: December 19, 2006
    Date of Patent: May 3, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Patent number: 7932039
    Abstract: The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.
    Type: Grant
    Filed: January 27, 2009
    Date of Patent: April 26, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Poonam Agarwal, Robert W. Kwiatkowski, Jr.