Patents Assigned to Third Wave Technologies
  • Target-dependent reactions using structure-bridging oligonucleotides
    Patent number: 6194149
    Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
    Type: Grant
    Filed: March 3, 1998
    Date of Patent: February 27, 2001
    Assignee: Third Wave Technologies, Inc.
    Inventors: Bruce Neri, Fang Dong, Victor Lyamichev, Mary Ann D. Brow, Lance Fors
  • Cleavage of nucleic acids
    Patent number: 6090543
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: December 2, 1996
    Date of Patent: July 18, 2000
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Cleavage agents
    Patent number: 6090606
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostabe organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: December 2, 1996
    Date of Patent: July 18, 2000
    Assignee: Third Wave Technologies, Inc.
    Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamicheva
  • Detection of nucleic acid sequences by invader-directed cleavage
    Patent number: 6001567
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.
    Type: Grant
    Filed: July 12, 1996
    Date of Patent: December 14, 1999
    Assignee: Third Wave Technologies, Inc.
    Inventors: Mary Ann D. Brow, Jeff Steven Grotelueschen Hall, Victor Lyamichev, David Michael Olive, James Robert Prudent
  • Detection of nucleic acids by multiple sequential invasive cleavages
    Patent number: 5994069
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
    Type: Grant
    Filed: March 24, 1997
    Date of Patent: November 30, 1999
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
  • Invasive cleavage of nucleic acids
    Patent number: 5985557
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: November 26, 1996
    Date of Patent: November 16, 1999
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Rapid detection and identification of nucleic acid variants
    Patent number: 5888780
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in nucleic acids. Methods are provided which allow for the identification of genetic mutations and the identification bacterial and viral strains and species in a sample.
    Type: Grant
    Filed: February 19, 1997
    Date of Patent: March 30, 1999
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow, Mary C. Oldenburg
  • Detection of nucleic acid sequences by invader-directed cleavage
    Patent number: 5846717
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: December 8, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: Mary Ann D. Brow, Jeff Steven Grotelueschen Hall, Victor Lyamichev, David Michael Olive, James Robert Prudent
  • Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
    Patent number: 5843669
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Structure-specific nucleases, including 5' nucleases, thermostable FEN-1 endonucleases and 3' exonucleases, are used to detect and identify target nucleic acids. Methods are provided which allow for the detection specific nucleic acid sequences; these methods permit the detection and identification of mutant and wild-type forms of genes (e.g., human genes) as well as permit the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: November 29, 1996
    Date of Patent: December 1, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamichev
  • Rapid detection of mutations in the p53 gene
    Patent number: 5843654
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in the human p53 gene. Methods are provided which allow for the identification of genetic mutations in the human p53 gene in a sample.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: December 1, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: Laura M. Heisler, Lance Fors, Mary Ann D. Brow
  • Detection of target nucleic acid molecules using thermostable 5' nuclease
    Patent number: 5837450
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: November 17, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Synthesis-deficient thermostable DNA polymerase
    Patent number: 5795763
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: August 18, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Cleavase fragment length polymorphism
    Patent number: 5719028
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: February 6, 1997
    Date of Patent: February 17, 1998
    Assignee: Third Wave Technologies Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow, Mary C. Oldenburg
  • 5' nucleases derived from thermostable DNA polymerase
    Patent number: 5614402
    Abstract: A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: June 6, 1994
    Date of Patent: March 25, 1997
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
    Patent number: 5541311
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
    Type: Grant
    Filed: June 4, 1993
    Date of Patent: July 30, 1996
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow