Abstract: A method is provided for determining in real time the course of proteolytic, e.g., thrombin activity, in a sample of clotting blood or plasma. By frequent mixing of a sample, clot formation may be controlled in a dense manner such that the majority of the sample remains fluid. This will inhibit cell precipitation and allow for informative monitoring as signal substrate is added.
Type:
Grant
Filed:
November 2, 2007
Date of Patent:
January 1, 2013
Assignee:
Thrombinoscope B.V.
Inventors:
Peter L. A. Giesen, Kerry Tappenden, Wynne Jones
Abstract: Methods and kits to determine thrombin activity are provided. They have the great advantage that there is no need to measure the whole calibrator curve in each individual medium, since a single-point measurement is adequate.
Type:
Grant
Filed:
June 6, 2007
Date of Patent:
March 13, 2012
Assignee:
Thrombinoscope B.V.
Inventors:
Peter L. A. Giesen, Timothy C. P. Van Asten
Abstract: A method is provided for determining in real time the course of thrombin activity in a sample of clotting blood or plasma as it appears in and disappears from the sample which comprises adding a signal substrate to said sample, said signal substrate causing a detectable signal related to the amount of conversion product formed upon reaction by the generated proteolytic activity, monitoring the signal development in time in said sample to provide a curve, and mixing said sample frequently so that clot formation occurs in a dense manner such that the majority of the sample remains fluid and that cell precipitation is inhibited, wherein said monitoring and mixing steps are repeated and performed in an alternate way.
Type:
Application
Filed:
November 2, 2007
Publication date:
April 29, 2010
Applicant:
THROMBINOSCOPE B.V.
Inventors:
Peter L.A. Giesen, Kerry Tappenden, Wynne Jones