Abstract: The present disclosure relates to an improved method for producing ergothioneine, comprising the steps of: (a) inoculating Pleurotus ostreatus strain CGMCC No. 6232 into a seed medium, and culturing it to prepare a seed liquor, wherein the seed medium uses soybean cake powder as nitrogen source; and (b) inoculating the seed liquor into a fermentation basal medium, and then culturing it to obtain a fermentation broth of Pleurotus ostreatus mycelia. Further, any one or more members selected from NH4Cl, NH4NO3, NaCl, polyethylene glycol, folic acid, vitamin B1 (VB1), indolebutyric acid, citric acid, pyruvic acid, arginine, lysine, leucine, aspartic acid, glutamic acid, betaine, histidine, cysteine, methionine, tween, span, chitosan, Fluconazole, Miconazole, Ketoconazole, ethylenediaminetetraacetic acid (EDTA), isopropyl alcohol and dimethyl sulfoxide are added into the fermentation basal medium.

Abstract: The present invention discloses a high concentration methanol tolerant methanotroph and its application, a accession number of the methanotroph in China General Microbiological Culture Collection Center being CGMCC No. 9873, deposit date being Oct. 29, 2014, category names being Methylomonas sp. ZR1. The methanotroph Methylomonas sp. ZR1 disclosed by the present invention can grow rapidly by using methane, and can tolerate with high concentration of methanol. The methanotroph Methylomonas sp. ZR1 can use C1 compounds such as methane and methanol to produce high value-added products such as carotenoids and polysaccharides, which has high application prospect in biological transformation of one-carbon chemistry.

Abstract: The invention relates to the field of modifying E. coli through genetic engineering. Specifically, the invention provides an E. coli containing a mutated lpdA gene. The invention also relates to use of the E. coli in the production of chemical material such as ethanol, and succinate etc. The invention also provides a method of producing chemical materials such as ethanol and succinate etc. by using the E. coli, as well as a method for increasing the activity of pyruvate dehydrogenase in E. coli by introducing a mutated lpdA gene.