Patents Assigned to TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
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Patent number: 11802280Abstract: An enzyme synthesizes hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde. Site-directed mutation of benzoylformate decarboxylase (BFD) creates a mutant of the enzyme, which can polymerize the formaldehyde, A phosphoketalose (F/XPK) generates acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone (DHA). Combination with phosphotransacetylase (Pta) provides a route from the formaldehyde to acetyl coenzyme A in three steps.Type: GrantFiled: February 12, 2018Date of Patent: October 31, 2023Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesInventors: Huifeng Jiang, Xiaoyun Lu, Yuwan Liu, Yiqun Yang, Lina Lu, Sheng Yang, Junran Gou
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Patent number: 11421199Abstract: Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of ?-elemene and/or germacrene A.Type: GrantFiled: November 2, 2017Date of Patent: August 23, 2022Assignees: Tianjin institute of Industrial Biotechnology, Chinese Academy of Sciences, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesInventors: Xueli Zhang, Luqi Huang, Zhubo Dai, Dong Wang, Lili Zhang, Juan Guo, Yi Liu
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Patent number: 11390890Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.Type: GrantFiled: July 20, 2020Date of Patent: July 19, 2022Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Chaoguang Tian, Jingen Li, Chuannan Long, Tao Sun, Liangcai Lin, Jing Xu, Qian Liu, Jingxiao Ji, Wenliang Sun, Yanhe Ma
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Patent number: 11306338Abstract: Disclosed is a new tagatose 6-phosphate 4-epimerase, which is capable of converting fructose 6-phosphate into tagatose 6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.Type: GrantFiled: December 28, 2018Date of Patent: April 19, 2022Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yanhe Ma, Yuanxia Sun, Yunjie Li, Jiangang Yang
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Publication number: 20210371844Abstract: The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.Type: ApplicationFiled: October 18, 2019Publication date: December 2, 2021Applicant: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Chaoguang TIAN, Junqi ZHAO, Jingen LI, Lu ZHANG
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Patent number: 10975400Abstract: A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.Type: GrantFiled: January 28, 2014Date of Patent: April 13, 2021Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesInventors: Ping Zheng, Jiuzhou Chen, Wei Pu, Jibin Sun, Xinyang Wu, Yanhe Ma
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Patent number: 10897057Abstract: The present invention is in the field of bioelectricity. The present invention provides energy generating systems, methods, and devices that are capable of converting chemical energy stored in sugars into useful electricity.Type: GrantFiled: September 19, 2018Date of Patent: January 19, 2021Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yi Heng Percival Zhang, Zhiguang Zhu
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Patent number: 10851334Abstract: Disclosed is a solid state biological reaction device, comprising a main tank (1), wherein the device further comprises a support (2) supporting the main tank (1), and the support (2) makes the main tank (1) be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.Type: GrantFiled: August 4, 2016Date of Patent: December 1, 2020Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesInventors: Wenxia Jiang, Xiaoran Zhang, Yanhe Ma
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Patent number: 10781462Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.Type: GrantFiled: February 4, 2016Date of Patent: September 22, 2020Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Chaoguang Tian, Jingen Li, Chuannan Long, Tao Sun, Liangcai Lin, Jing Xu, Qian Liu, Jingxiao Ji, Wenliang Sun, Yanhe Ma
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Patent number: 10736970Abstract: The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.Type: GrantFiled: July 25, 2017Date of Patent: August 11, 2020Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Peijian Zou, Huanbo Tan, Wencheng Su, Jibin Sun
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Patent number: 10316303Abstract: A lactone hydrolase having improved activity towards ?-zearalenol is disclosed. The lactone hydrolase has a modified amino acid sequence of SEQ ID NO: 5, wherein the modification is a substitution of valine at position 167 with histidine. A method of degrading ?-zearalenol using such lactone hydrolase is also disclosed.Type: GrantFiled: December 1, 2016Date of Patent: June 11, 2019Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Rey-Ting Guo, Yingying Zheng, Zhongxia Xu, Chun-Chi Chen, Wei Peng, Weidong Liu, Je-Ruei Liu, Yanhe Ma
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Patent number: 10260079Abstract: The present disclosure relates to an improved method for producing ergothioneine, comprising the steps of: (a) inoculating Pleurotus ostreatus strain CGMCC No.6232 into a seed medium, and culturing it to prepare a seed liquor, wherein the seed medium uses soybean cake powder as nitrogen source; and (b) inoculating the seed liquor into a fermentation basal medium, and then culturing it to obtain a fermentation broth of Pleurotus ostreatus mycelia. Further, any one or more members selected from NH4Cl, NH4NO3, NaCl, polyethylene glycol, folic acid, vitamin B1 (VB1), indolebutyric acid, citric acid, pyruvic acid, arginine, lysine, leucine, aspartic acid, glutamic acid, betaine, histidine, cysteine, methionine, tween, span, chitosan, Fluconazole, Miconazole, Ketoconazole, ethylenediaminetetraacetic acid (EDTA), isopropyl alcohol and dimethyl sulfoxide are added into the fermentation basal medium.Type: GrantFiled: May 29, 2015Date of Patent: April 16, 2019Assignees: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES, TIANJIN SINONOCY BIOLOGICAL TECHNOLOGY CO., LTD.Inventors: Wenxia Jiang, Qi Liu, Weiya Zhang, Baoliang Mei
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Patent number: 10128522Abstract: The present invention is in the field of bioelectricity. The present invention provides energy generating systems, methods, and devices that are capable of converting chemical energy stored in sugars into useful electricity.Type: GrantFiled: June 5, 2014Date of Patent: November 13, 2018Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yi Heng Percival Zhang, Zhiguang Zhu
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Publication number: 20180273894Abstract: The disclosure relates to the biotechnology field, and a method for preparing degradable materials, which comprises: inoculating Ceriporia lacerata into a culture medium containing lignocellulose debris for solid-state fermentation. The accession number of the C. lacerata is CGMCC No. 10485. The disclosure also relates to a degradable material obtained by the method and a method for cultivating the C. lacerata. The mycelia of the C. lacerata used herein can enwind and fix the culture medium debris (a lignocellulose material). By dint of the C. lacerata, the lignocellulose material can be prepared into a degradable material without sterilization or anti-bacterial treatment, and the preparation process doesn't require a sterile environment; thus, the preparation cost is decreased remarkably.Type: ApplicationFiled: March 21, 2017Publication date: September 27, 2018Applicant: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Wenxia Jiang, Ping Yang, Guobing Shao, Qi Liu
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Patent number: 10006063Abstract: The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.Type: GrantFiled: May 23, 2014Date of Patent: June 26, 2018Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Xueli Zhang, Xinna Zhu, Hongtao Xu, Zaigao Tan
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Publication number: 20180163238Abstract: Disclosed is a preparation containing ergothioneine, the ergothioneine is provided by an extracellular ferment liquor which is obtained by removing the mycelia from the ferment liquor produced through liquid fermentation of a mushroom and/or the concentrate of the extracellular ferment liquor, and the preparation comprises food, cosmetics and animal feed. The method for preparing the preparation comprises liquid fermentation of a mushroom, removing the mycelia from the fermentation product, and selectively concentrating the extracellular ferment liquor obtained after removing the mycelia. The extracellular ferment liquor obtained through the liquid fermentation of a mushroom and/or the concentrate of the extracellular ferment liquor are used in preparing the preparation containing ergothioneine.Type: ApplicationFiled: May 29, 2015Publication date: June 14, 2018Applicants: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCE, TIANJIN SINONOCY BIOLOGICAL TECHNOLOGY CO., LTD.Inventors: Wenxia Jiang, Ping Yang, Qi Liu, Weiya Zhang, Tao Zhou, Baoliang Mei, Zizhen Zhou, Miao Li, Dalong Zhang
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Patent number: 9944957Abstract: Provided is recombinant Escherichia coli comprising a mutated ldhA gene. Disclosed are use of the recombinant Escherichia coli in the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant Escherichia coli. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50° C.), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.Type: GrantFiled: July 9, 2014Date of Patent: April 17, 2018Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yanhe Ma, Xueli Zhang, Hongtao Xu, Xinna Zhu, Pingping Liu, Jinlei Tang
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Patent number: 9896734Abstract: Provided in the present invention is an asparaginic acid kinase, and the 340th amino acid residue in the position of the amino acid sequence of the asparaginic acid kinase corresponding to the amino acid sequence shown in SEQ ID NO: 2 is a non-aspartic acid. The asparaginic acid of the present invention can efficiently relieve the feedback inhibition of L-lysine, and can be effectively used for the production of L-lysine. Also provided in the present invention are host cells comprising genes coding the asparaginic acid kinase and a method for producing L-lysine using the host cells or the asparaginic acid kinase. The asparaginic acid kinase of the present invention or the host cells comprising the asparaginic acid kinase of the present invention is also used to produce L-threonine, L-methionine, L-isoleucine or L-valine. Also provided in the present invention is a method of producing L-aspartyl-4-yl phosphoric acid using the asparaginic acid kinase or the host cells.Type: GrantFiled: May 16, 2013Date of Patent: February 20, 2018Assignees: Cofco Biochemical (Anhui) Co., Ltd., Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesInventors: Guojun Yue, Jibin Sun, Ping Zheng, Jiao Liu, Qinggang Li, Linghe Xia, Yongsheng Zhou, Hu Luo, Yong Zhou, Yun Man, Zongmei Lu, Yanhe Ma
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Publication number: 20170360949Abstract: The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.Type: ApplicationFiled: July 25, 2017Publication date: December 21, 2017Applicant: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Peijian ZOU, Huanbo TAN, Wencheng SU, Jibin SUN
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Patent number: 9816124Abstract: Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of ?-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof. The method for improving the enzymatic activity of ?-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in escherichia coli or the mutant thereof with any of the following regulating elements: artificial regulating element M 1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria.Type: GrantFiled: January 28, 2014Date of Patent: November 14, 2017Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesInventors: Xueli Zhang, Qingyan Li, Jing Zhao, Tao Sun, Guanping Dai, Hongtao Xu, Jinlei Tang, Yanhe Ma