Patents Assigned to Tianjin University of Science and Technology
  • Publication number: 20210355457
    Abstract: The present disclosure provides recombinant Pichia pastoris, a construction method thereof, and use thereof in the efficient preparation of 15?-D-ethylgonendione, and belongs to the technical field of fermentation engineering. In the present disclosure, with Pichia pastoris as a host, 15?-steroid hydroxylase and glucose-6-phosphate dehydrogenase (G6PD) are simultaneously induced in a manner of intracellular enzyme production for the first time, and the Pichia pastoris whole-cell induction and catalysis mode is used to achieve the conversion of D-ethylgonendione with high substrate loading and high conversion rate.
    Type: Application
    Filed: May 12, 2021
    Publication date: November 18, 2021
    Applicant: Tianjin University of Science and Technology
    Inventors: Xiaoguang Liu, Fuping Lu, Shuhong Mao, Peng Jin, Dongqi Xie, Yanxi Chen, Jinhong Li
  • Patent number: 11124780
    Abstract: The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome of E coli prompted by strong promoter Ptrc to reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Ptrc on the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.
    Type: Grant
    Filed: May 12, 2019
    Date of Patent: September 21, 2021
    Assignee: Tianjin University of Science and Technology
    Inventors: Xixian Xie, Ning Chen, Heyun Wu, Guoliang Li, Qiang Li, Xiaoguang Fan, Qingyang Xu, Chenglin Zhang, Yanjun Li, Qian Ma
  • Publication number: 20210214509
    Abstract: A lignocellulose nanofibril material, a stable foam system based thereon, a preparation method and an application thereof are provided. The lignocellulosic nanofibril material includes the following components: 0.5-20 wt % of wood flour, 0.1-10 wt % of (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl, 2-25 mmol/g of an oxidant, 6-15 wt % of NaBr, and the remaining is water. The stable foam system based on the lignocellulosic nanofibril material includes: 0.1-1.0 wt % of the lignocellulosic nanofibril material, 0.2-1.0 wt % of a surfactant, 0.1-10 wt % of sodium chloride, 0.1-1.0 wt % of calcium chloride, 0.1-1.0 wt % of magnesium chloride, 0.1-1.0 wt % of sodium sulfate, and a balance of water.
    Type: Application
    Filed: April 15, 2019
    Publication date: July 15, 2021
    Applicants: Southwest Petroleum University, Tianjin University of Science and Technology
    Inventors: Bing WEI, Yangbing WEN, Yuanyuan WANG, Wanfen PU, Shengen CHEN
  • Publication number: 20210198679
    Abstract: A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.
    Type: Application
    Filed: December 25, 2020
    Publication date: July 1, 2021
    Applicant: Tianjin University of Science and Technology
    Inventors: Yefu CHEN, Yanrui MA, Yongjing DU, Sen JIANG, Jinying REN, Guo ZHANG, Xinyue KANG, Peng ZHENG, Xiaole WU, Dongguang XIAO, Xuewu GUO
  • Publication number: 20210024942
    Abstract: An Aureobasidium pullulans recombinant strain with high-yield heavy oil and a construction method and application thereof are provided. The Aureobasidium pullulans recombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtained Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.
    Type: Application
    Filed: October 15, 2020
    Publication date: January 28, 2021
    Applicant: Tianjin University of Science and Technology
    Inventors: Yefu CHEN, Siyao HUANG, Peng ZHENG, Yuanhua WANG, Jian GUO, Mengjuan ZHANG, Xuewu GUO, Dongguang XIAO
  • Patent number: 10472654
    Abstract: Disclosed are very high optically pure D- and L-lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L-lactic acids using the strains, wherein the deposit number of the D-lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L-lactic acid fermentation production strain is CGMCC No. 11060.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: November 12, 2019
    Assignee: Tianjin University of Science and Technology
    Inventors: Zhengxiang Wang, Kangming Tian, Dandan Niu, Fuping Lu