Patents Assigned to Tianjin University of Science and Technology
-
Publication number: 20210355457Abstract: The present disclosure provides recombinant Pichia pastoris, a construction method thereof, and use thereof in the efficient preparation of 15?-D-ethylgonendione, and belongs to the technical field of fermentation engineering. In the present disclosure, with Pichia pastoris as a host, 15?-steroid hydroxylase and glucose-6-phosphate dehydrogenase (G6PD) are simultaneously induced in a manner of intracellular enzyme production for the first time, and the Pichia pastoris whole-cell induction and catalysis mode is used to achieve the conversion of D-ethylgonendione with high substrate loading and high conversion rate.Type: ApplicationFiled: May 12, 2021Publication date: November 18, 2021Applicant: Tianjin University of Science and TechnologyInventors: Xiaoguang Liu, Fuping Lu, Shuhong Mao, Peng Jin, Dongqi Xie, Yanxi Chen, Jinhong Li
-
Patent number: 11124780Abstract: The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome of E coli prompted by strong promoter Ptrc to reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Ptrc on the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.Type: GrantFiled: May 12, 2019Date of Patent: September 21, 2021Assignee: Tianjin University of Science and TechnologyInventors: Xixian Xie, Ning Chen, Heyun Wu, Guoliang Li, Qiang Li, Xiaoguang Fan, Qingyang Xu, Chenglin Zhang, Yanjun Li, Qian Ma
-
Publication number: 20210214509Abstract: A lignocellulose nanofibril material, a stable foam system based thereon, a preparation method and an application thereof are provided. The lignocellulosic nanofibril material includes the following components: 0.5-20 wt % of wood flour, 0.1-10 wt % of (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl, 2-25 mmol/g of an oxidant, 6-15 wt % of NaBr, and the remaining is water. The stable foam system based on the lignocellulosic nanofibril material includes: 0.1-1.0 wt % of the lignocellulosic nanofibril material, 0.2-1.0 wt % of a surfactant, 0.1-10 wt % of sodium chloride, 0.1-1.0 wt % of calcium chloride, 0.1-1.0 wt % of magnesium chloride, 0.1-1.0 wt % of sodium sulfate, and a balance of water.Type: ApplicationFiled: April 15, 2019Publication date: July 15, 2021Applicants: Southwest Petroleum University, Tianjin University of Science and TechnologyInventors: Bing WEI, Yangbing WEN, Yuanyuan WANG, Wanfen PU, Shengen CHEN
-
Publication number: 20210198679Abstract: A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.Type: ApplicationFiled: December 25, 2020Publication date: July 1, 2021Applicant: Tianjin University of Science and TechnologyInventors: Yefu CHEN, Yanrui MA, Yongjing DU, Sen JIANG, Jinying REN, Guo ZHANG, Xinyue KANG, Peng ZHENG, Xiaole WU, Dongguang XIAO, Xuewu GUO
-
Publication number: 20210024942Abstract: An Aureobasidium pullulans recombinant strain with high-yield heavy oil and a construction method and application thereof are provided. The Aureobasidium pullulans recombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtained Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.Type: ApplicationFiled: October 15, 2020Publication date: January 28, 2021Applicant: Tianjin University of Science and TechnologyInventors: Yefu CHEN, Siyao HUANG, Peng ZHENG, Yuanhua WANG, Jian GUO, Mengjuan ZHANG, Xuewu GUO, Dongguang XIAO
-
Patent number: 10703717Abstract: A novel type of water-soluble isatin derivatives, and manufacturing method and application thereof. An isatin derivative comprising a phenolic hydroxy group is used as the substrates. A dimethylaminomethylene group is introduced to an ortho position of the phenolic hydroxy group to significantly improve the water solubility of a class of compounds provided by the invention. An antitumor activity study showed that the activity of the class of compounds was not reduced, and even improved. The class of compounds has great prospects for applications in developing an antitumor pharmaceutical product.Type: GrantFiled: July 28, 2016Date of Patent: July 7, 2020Assignee: Tianjin University of Science & TechnologyInventors: Peng Yu, Dong Wang, Yuou Teng, Longfei Miao, Jing Wang, Qian Zhang, Yuan Yuan
-
Publication number: 20200181844Abstract: The present invention provides reconstituted tobacco and a preparation method thereof, and belongs to the technical field of tobacco. The preparation method provided by the present invention includes the steps of: subjecting tobacco stems to hot-water extraction and pulping by mill successively to obtain tobacco stem pulp; mixing the tobacco stem pulp with alkaline matter for oxygen delignification in an oxygen environment to obtain oxygen-delignified tobacco stem pulp, where the alkaline matter includes one or more of sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate and sodium bicarbonate; mixing the oxygen-delignified tobacco stem pulp with tobacco pulp, calcium carbonate and a retention agent, and subjecting the mixture to a papermaking process in the paper machine to obtain reconstituted tobacco sheets; and coating the reconstituted tobacco sheets with a concentrated tobacco extracting solution to obtain reconstituted tobacco.Type: ApplicationFiled: December 11, 2018Publication date: June 11, 2020Applicants: HENAN CIGARETTE INDUSTRY TOBACCO SHEET., LTD, Tianjin University of Science & TechnologyInventors: Chaowei WU, Haijian YANG, Chong LUO, Yu SUN, Jianhong ZHANG, Xiangfan WANG, Xiaofei WANG, Yangbing WEN
-
Publication number: 20200178590Abstract: The present invention provides a preparation method of tobacco sheets with a reduced quantity of wood pulp fibers, and belongs to the technical field of reconstituted tobacco. The method includes the steps of: cooking tobacco stem materials by alkaline process, and filtering and washing the cooked tobacco stem materials to obtain tobacco stem fibers; generating mixed pulp by mixing wood pulp fibers, tobacco pulp, filling materials, water and the tobacco stem fibers together as per certain proportions, and subjecting the mixed pulp to a papermaking process in a paper machine to obtain tobacco sheets. By means of adding tobacco stem fibers, the present invention effectively reduces the quantity of wood pulp fibers, ensures the continuous operation of the paper machine, and effectively improves the bulk and tensile index of prepared tobacco sheets.Type: ApplicationFiled: December 11, 2018Publication date: June 11, 2020Applicants: Henan Cigarette Industry Tobacco Sheet., Ltd, Tianjin University of Science & TechnologyInventors: Chaowei Wu, Wenling Wang, Xinshun Fan, Xiaohui Tian, Haijian Yang, Chong Luo, Yanqing Wang, Yangbing Wen
-
Publication number: 20190359568Abstract: A novel type of water-soluble isatin derivatives, and manufacturing method and application thereof. An isatin derivative comprising a phenolic hydroxy group is used as the substrates. A dimethylaminomethylene group is introduced to an ortho position of the phenolic hydroxy group to significantly improve the water solubility of a class of compounds provided by the invention. An antitumor activity study showed that the activity of the class of compounds was not reduced, and even improved. The class of compounds has great prospects for applications in developing an antitumor pharmaceutical product.Type: ApplicationFiled: July 28, 2016Publication date: November 28, 2019Applicant: Tianjin University of Science & TechnologyInventors: Peng YU, Dong WANG, Yuou TENG, Longfei MIAO, Jing WANG, Qian ZHANG, Yuan YUAN
-
Publication number: 20190358219Abstract: Disclosed is a targeted delivery system for a hydrophobic antitumor drug, referring to conjugates of E-selectin polypeptide ligand-polyethylene glycol-antitumor drug connected by different link bridges containing disulfide bonds. The synthesis of the conjugates, antitumor activity evaluation, the particle size and morphology characteristics of nanoparticles self-assembled by the conjugates in an aqueous solution, and the release of the antitumor drug in different conditions are comprised. The conjugates can actively target at vessels of a tumor site by the E-selectin peptide ligand, and can also self-assemble into nanoparticles in an aqueous solution, so as to be passively targeted at the tumor site by EPR effect.Type: ApplicationFiled: July 21, 2016Publication date: November 28, 2019Applicant: Tianjin University of Science & TechnologyInventors: Peng YU, Na GUO, Dong WANG, Yuou TENG, Tiantian HAO, Huan LIU, Tianle ZHANG, Xiuzhuan SHANG
-
Patent number: 10472654Abstract: Disclosed are very high optically pure D- and L-lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L-lactic acids using the strains, wherein the deposit number of the D-lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L-lactic acid fermentation production strain is CGMCC No. 11060.Type: GrantFiled: November 20, 2017Date of Patent: November 12, 2019Assignee: Tianjin University of Science and TechnologyInventors: Zhengxiang Wang, Kangming Tian, Dandan Niu, Fuping Lu
-
Publication number: 20120255707Abstract: An inflatable wall material, including: a thermal insulation layer, a first metal layer, a barrier layer, a buffer layer, a protective layer, and a second metal layer. The first metal layer, the barrier layer, the buffer layer, the protective layer, and the second metal layer are arranged on two sides of the thermal insulation layer in sequence from the inside to the outside. The two barrier layers on the two sides of the thermal insulation layer employ air-tight materials. The two barrier layers and the first metal layers on the two sides of the thermal insulation layer constitute an inflatable air-tight space. The thermal insulation layer is positioned in the air-tight space.Type: ApplicationFiled: June 12, 2012Publication date: October 11, 2012Applicants: TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY, Tianjin Hongmin Institute of Mini Cold Storage Room, Tianjin Gasin-DH Preservation Technologies Co., Ltd., Tianjin Lvxin Lower Temperature Technology & Science Co., Ltd.Inventors: Xihong LI, Yunbin JIANG, Yage XING, Shulai LU, Xiufeng LIU, Weili LI, Chongxiao SHAO, Haidong LIU