Abstract: The present disclosure relates to novel microfluidic devices and their use for operating microphysiological systems. The novel microfluidic devices are particularly useful for pharmacokinetic-pharmacodynamic analysis in the context of ADME(T) profiling. The present disclosure provides novel microfluidic devices comprising a first “blood” circuit with one or more cell culture compartments, and a second “urine” circuit comprising a renal filtration and reabsorption unit, wherein both circuits are connected with each other via the filtration unit and the reabsorption unit. The present disclosure further provides methods, kits and assays implementing the novel microfluidic devices.

Abstract: The present disclosure relates to novel multi-organ-chips establishing the differentiation of induced pluripotent stem cell (iPSC)-derived cells into organ equivalents on microfluidic devices and corresponding methods of generating organ equivalents. The present disclosure also relates to novel bioengineered tissue constructs mimicking organ barriers generated with iPSC-derived endothelial cells and/or organoids bioprinted in, and/or seeded on, a hydrogel. The present disclosure further relates to methods of bio-engineering organ constructs comprising co-culturing iPSC-derived organ precursor cells and iPSC-derived fibroblasts and endothelial cells.

Abstract: A 3D in vitro bi-phasic cartilage-bone organoid includes a layer of an artificial cartilage tissue, and a layer of an artificial bone tissue comprising a structure-giving scaffold and a bone marrow structure. The layer of the artificial cartilage tissue contacts at least one surface of the layer of the artificial bone tissue.

Abstract: The present invention is directed to a multi-organ-chip device comprising a base layer; an organ layer arranged on the base layer; an antra layer arranged on the organ layer; and an actuator layer; wherein the base layer is configured to provide a solid support for the further layers; the organ layer is configured to comprise a multiplicity of individual organ equivalents, each organ equivalent comprising one or more organ growth sections, each of the organ growth sections being configured to comprise an organoid cavity for housing at least one organoid of an organ and to comprise a micro-inlet and a micro-outlet for fluid communication between the organoid cavity of the organ growth section and a self-contained circulation system, wherein the organ layer comprises at least one organ equivalent configured to represent the organs lung, small intestine, spleen, pancreas, liver, kidney and bone marrow, respectively, and a self-contained circulation system configured to be in direct fluid communication with the o

Abstract: A self-contained circulation system, which supports the formation of capillaries in capillary growth sections and allows the formation of micro organoids and/or micro tissue sections for monitoring the effect of one or more test compounds and determining efficacy, side-effects, biosafety, metabolites, mode of action or organ regeneration as well as methods of establishing such micro organoids and/or micro tissue in the self-contained circulation system.

Abstract: The present invention is directed to a multi-organ-chip device comprising a base layer; an organ layer arranged on the base layer; an antra layer arranged on the organ layer; and an actuator layer; wherein the base layer is configured to provide a solid support for the further layers; the organ layer is configured to comprise a multiplicity of individual organ equivalents, each organ equivalent comprising one or more organ growth sections, each of the organ growth sections being configured to comprise an organoid cavity for housing at least one organoid of an organ and to comprise a micro-inlet and a micro-outlet for fluid communication between the organoid cavity of the organ growth section and a self-contained circulation system, wherein the organ layer comprises at least one organ equivalent configured to represent the organs lung, small intestine, spleen, pancreas, liver, kidney and bone marrow, respectively, and a self-contained circulation system configured to be in direct fluid communication with the o

Abstract: A 3D in vitro bi-phasic cartilage-bone organoid includes a layer of an artificial cartilage tissue, and a layer of an artificial bone tissue comprising a structure-giving scaffold and a bone marrow structure. The layer of the artificial cartilage tissue contacts at least one surface of the layer of the artificial bone tissue.

Abstract: A method for at least one of determining and monitoring at least one condition of/in a three dimensional cell culture system comprising at least one growth section, the at least one condition being selected from the group consisting of a physiological condition, a vitality, and a metabolism status, the method includes determining the physiological condition using erythrocytes as detectors, determining and/or monitoring the vitality by measuring living cell fluorescent dyes, and determining and/or monitoring the metabolism status by at least one of measuring an autofluorescence of NADH and/or FAD, by determining a NADH/NAD+ ratio, and by determining a NADH/FAD ratio.