Abstract: A chip includes a micro-channel unit for hydraulically classifying cells in a blood sample. In a micro-channel unit, liquid flowing from a sub channel into a main channel pushes cells flowing in the main channel toward a side thereof on which a removal channel and a collection channel are disposed. Fluid containing non-nucleated RBCs among the pushed cells enters the removal channel, so that the non-nucleated RBCs are removed from a blood sample. A plurality of micro-channel units having the same patterns as each other are repeatedly stacked in a height direction. Inlets of the main channels, inlets of the sub channels, outlets of the removal channels, outlets of the collection channels, and outlets of the main channels, which are provided in the micro-channel units, are connected to respective pillar channels penetrating each of layers in a traversing manner.

Abstract: In the present invention, a fraction (A) is labeled, the fraction (A) being a fraction obtained from a maternal blood sample and in which nucleated red blood cells (NRBCs) are concentrated in a population of whole blood cells. Then, a fraction (B) having increase purity of NRBCs is obtained by sorting out blood cells in the labeled fraction A by at least cell sorting. Next, fractions (C) are obtained by separating each blood cell in the fraction (B) at a single-cell level and independently performing a process for extracting a nucleic acid for each separated blood cell, each of the fractions (C) containing a nucleic acid distinguishable at a single-cell level. Then, a fraction (D) containing a nucleic acid derived from a fetus is sorted out from a group of fractions (C) by performing a molecular biological analysis for each of the fractions (C).

Abstract: Blood containing cells is brought into contact with a porous surface of a porous material before classification of the cells in the blood by flowing the blood through a microchannel. In an example, the porous material is added to the blood containing the cells and mixed together, thereby bringing the blood containing the cells into contact with the porous surface. In an example, the porous material has particles with the porous surface including polysaccharides. The porous material is added to the blood containing the cells while being suspended in a liquid. In an example, the particles have a predetermined particle size distribution. A median particle size d50V in the volume-based cumulative distribution is 25 to 280 ?m.

Abstract: A chip includes a micro-channel unit for hydraulically classifying cells in a blood sample. In a micro-channel unit, liquid flowing from a sub channel into a main channel pushes cells flowing in the main channel toward a side thereof on which a removal channel and a collection channel are disposed. Fluid containing non-nucleated RBCs among the pushed cells enters the removal channel, so that the non-nucleated RBCs are removed from a blood sample. A plurality of micro-channel units having the same patterns as each other are repeatedly stacked in a height direction. Inlets of the main channels, inlets of the sub channels, outlets of the removal channels, outlets of the collection channels, and outlets of the main channels, which are provided in the micro-channel units, are connected to respective pillar channels penetrating each of layers in a traversing manner.

Abstract: In the present invention, a fraction (A) is labeled, the fraction (A) being a fraction obtained from a maternal blood sample and in which nucleated red blood cells (NRBCs) are concentrated in a population of whole blood cells. Then, a fraction (B) having increase purity of NRBCs is obtained by sorting out blood cells in the labeled fraction A by at least cell sorting. Next, fractions (C) are obtained by separating each blood cell in the fraction (B) at a single-cell level and independently performing a process for extracting a nucleic acid for each separated blood cell, each of the fractions (C) containing a nucleic acid distinguishable at a single-cell level. Then, a fraction (D) containing a nucleic acid derived from a fetus is sorted out from a group of fractions (C) by performing a molecular biological analysis for each of the fractions (C).