Abstract: Disclosed herein, in certain embodiments, are vector-free methods of manufacturing engineered immune cells. In some embodiments, also disclosed herein are compositions comprising engineered immune cells obtained from the methods and processes described herein. In additional embodiments, disclosed herein are methods of treating a disease and kits using engineered immune cells obtained from the methods and processes described herein.

Abstract: Described are improved methods for manufacturing and purifying clinical-grade retroviral vectors, such as lentiviral vectors.

Abstract: The present invention provides gene edited modified immune cells suitable for adoptive T cell therapy comprising a nucleic acid capable of downregulating CD3?, CD3?, CD3?, B2M, CIITA, TAP1, TAP2, TAPBP, NLRC5, HLA-DM, RFX5, RFXANK, RFXAP, and invariant chain; and further comprising an exogenous nucleic acid encoding a chimeric antigen receptor (CAR), an engineered T cell receptor (TCR), a Killer cell immunoglobulin-like receptor (KIR), dominant negative receptor and/or a switch receptor. Also provided are compositions and methods for generating the modified immune cell, and methods of using the modified immune cells for adoptive therapy and treating a disease or condition.

Abstract: The present disclosure provides methods for the administration of engineered cells, such as T cells, to subjects for adoptive cell therapy. Also provided are compositions and articles of manufacture for use in the methods. The cells express chimeric antigen receptors (CARs) and/or T cell receptors (TCRs), and optionally, other molecules to overcome the immunosuppressive tumor microenvironment. Methods provided herein may employ a fractionated dosing regimen which may further comprise monitoring the development of a toxicity and managing the symptoms thereof.