Abstract: A deletion in the end region of the long arm of a Chromosome 9 is efficiently detected. A genetic testing kit of bladder cancer according to the present invention includes a primer allowing for efficient amplification of a region containing a site of genetic polymorphism present in the ABO blood group gene of Chromosome 9. In the site of genetic polymorphism present in the ABO blood group gene, the frequency of heterozygote (heterozygosity) in the population is extremely high. Therefore, by detecting LOH using a polymorphic site present in the ABO blood group gene, it is possible to reliably detect a deletion near the polymorphic site, in other words, a deletion near the end of the long arm of Chromosome 9.
Abstract: There is provided a method of detecting or analyzing nucleic acid with a high reliability and reproducibility, capable of sampling target nucleic acid from initial target nucleic acid having not less than two types of sequences different from each other in a ratio which is proportional to that of initial target nucleic acid. A method of detecting or analyzing nucleic acid by sampling target nucleic acid from not less than two types of target nucleic acid samples having base sequences different in at least one base, and subjecting the sampled nucleic acid sample to detection or analysis; comprising steps of: obtaining the number of copies of initial target nucleic acid from the concentration of a nucleic acid in an initial target nucleic acid sample; and sampling the target nucleic acid of a predetermined number or more of copies from the initial target nucleic acid sample.
Abstract: A process for biologically preventing dicotyledonous plant diseases which comprises: cutting a seedling of a dicotyledon which includes the seedling between a cotyledon and less than three leaves at a growth stage; immersing the upper portion of the cut seedling into a symbiotical bacteria suspension having antifungal and antibacterial activities and induced resistance to plant pathogens in order to inoculate the symbiotical bacteria into interior tissues in the vessel and intercellular space of the dicotyledonous plants; cutting the seedlings in a nursery bed or directly planting them in a field for further association of the symbiotical bacteria; and preventing dicotyledonous plant diseases of the dicotyledonous plants.
Abstract: A new Pseudomonas gladioli having the identifying characteristics of Bikohken-kin No. 8805 has been discovered. The microorganism is a new bacteria separated from a bulb and roots of Miltonia. For separation, the bulb and roots of Miltonia are ground in a 1% solution of peptone followed by a streak culture on a bouillon agar at 25.degree. C. for 48-96 hours, and the colonies thus grown are isolated. This microorganism is inoculated into a bulb and roots of a plant selected from the group consisting of Welsh onion, sorgo, oats and maize. The plants inoculated with the grown microorganisms are grown together within the radius of rhizosphere of a plant to be protected (or companion or mixed crop) for further multiplication of Pseudomonas gladioli M-2196 in order to control soil borne plant diseases caused by Fusarium oxysporum. Very strong antibacterial activity on Fusarium oxysporum, Rhizoctonia solani, Verticillum dahliae, Corynebacterium michiganese pv. michiganese, Sclerotium cepivorum etc. is observed.