Abstract: In a first step, when a ?-1,6-branched ?-1,3-glucan is contained in a test specimen, an active reporter protein comprising one and the other of split reporter proteins is formed by binding the ?-1,6-branched ?-1,3-glucan to a first fusion protein and a second fusion protein, and when a ?-1,3-glucan is contained in the test specimen, the active reporter protein comprising one and the other of the split reporter proteins is formed by binding the ?-1,3-glucan to the first fusion protein and a third fusion protein, and in a second step, the active reporter protein formed in the first step is detected and quantified.

Abstract: The ?-glucan-binding protein contains an amino acid sequence represented by SEQ ID NO: 1.

Abstract: A ?-1,6-glucanase mutant which is a mutant of ?-1,6-glucanase (EC 3.2.1.75), wherein a Glu residue located at a position corresponding to Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X or a Glu (E) residue located at a position corresponding to each of Glu (E)-225 and Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X, wherein the amino acid residue (X) is selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H); and a method for measuring ?-1,6-glucan, including measuring ?-1,6-glucan bonded to the mutant.

Abstract: A ?-1,6-glucanase mutant which is a mutant of ?-1,6-glucanase (EC 3.2.1.75), wherein a Glu residue located at a position corresponding to Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X or a Glu (E) residue located at a position corresponding to each of Glu (E)-225 and Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X, wherein the amino acid residue (X) is selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H); and a method for measuring ?-1,6-glucan, including measuring ?-1,6-glucan bonded to the mutant.