Patents Assigned to Tokai University Educational System
  • Patent number: 11892456
    Abstract: A marker for examining a diabetic complication comprising a compound represented by the following Formula (1), or a salt thereof.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: February 6, 2024
    Assignees: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, RIKEN
    Inventors: Ryoji Nagai, Naoyuki Taniguchi
  • Patent number: 11732273
    Abstract: Disclosed are methods and compositions for in situ germline genome engineering. The disclosed methods and compositions may be utilized for germline genome engineering in a subject having a reproductive organ containing a fertilized zygote, via: (i) isolating or obtaining the reproductive organ from the subject after a time period following insemination of the subject; (ii) introducing a reagent composition into the reproductive organ, the reagent composition comprising a nuclease system and/or an exogeneous polynucleotide; and (iii) electroporating the reproductive organ.
    Type: Grant
    Filed: February 24, 2020
    Date of Patent: August 22, 2023
    Assignees: Board of Regents of the University of Nebraska, Tokai University Educational System
    Inventors: Channabasavaiah Gurumurthy, Masato Ohtsuka, Masahiro Sato
  • Publication number: 20230167409
    Abstract: A method of culturing a cell population containing cartilage-derived cells positive for expression of Tie2 (cartilage-derived Tie2-positive cells), the method including culturing a cell population containing cartilage-derived Tie2-positive cells in a culture medium containing at least one kind of Tie2 expression enhancer other than growth factors (e.g., an extract derived from a plant of the genus Cinnamomum). This culturing method is preferably performed in cultureware having a culture surface coated with a coating agent (e.g., a polylysine-containing agent).
    Type: Application
    Filed: April 26, 2021
    Publication date: June 1, 2023
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, NIPPON ZOKI PHARMACEUTICAL CO., LTD.
    Inventors: Daisuke SAKAI, Yoshihiko NAKAMURA, Erika MATSUSHITA
  • Publication number: 20230109743
    Abstract: A fluorescence guide plate includes first and second surfaces, an edge surface connecting a periphery of the first surface with a periphery of the second surface, and a dichroic mirror laminated on the first surface. Fluorescent material is dispersed at least one of inside a space defined by the first surface, the second surface, and the edge surface, on the first surface, or on the second surface. The fluorescence guide plate has a plate-shaped structure made of a material with a higher refractive index than an outside. The fluorescence guide plate is configured such that, when irradiation light enters from the first surface, the fluorescence emitted from the fluorescent material exits from the edge surface. A reflection wavelength band of a normal incident beam reflected by the dichroic mirror lies in a range of wavelengths longer than a peak wavelength of a fluorescence wavelength band of the fluorescent material.
    Type: Application
    Filed: August 23, 2022
    Publication date: April 13, 2023
    Applicants: TOYOTA JIDOSHA KABUSHIKI KAISHA, TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Taizo Masuda, Masamori Endo
  • Patent number: 11549126
    Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: January 10, 2023
    Assignees: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA, TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Channabasavaiah B. Gurumurthy, Hiromi Miura, Masato Ohtsuka
  • Publication number: 20220370380
    Abstract: [Problem] To provide a composition or the like that suppresses the physical destruction of red blood cells (hemolysis) due to exercises and behaviors associated with impact such as striking, for example, as when continually hitting the soles of the feet on the ground by continued running, by using astaxanthin which has a long history of use as a food, and to provide a composition or the like for making the number of red blood cells destroyed (hemolysis number) to lower than the number of red blood cells newly created by hematopoiesis (hematopoiesis number), to suppress and/or improve exercise-induced hemolytic anemia caused by the physical destruction of red blood cells (hemolysis). [Solution] Astaxanthin is used as an active ingredient.
    Type: Application
    Filed: October 29, 2020
    Publication date: November 24, 2022
    Applicants: AstaReal Co., Ltd., Tokai University Educational System
    Inventors: Nobuko Hongo, Rika Hirashima, Kumi Tominaga, Yasuhiro Nishizaki
  • Publication number: 20220364086
    Abstract: The present invention aims at establishing a novel therapy for facioscapulohumeral muscular dystrophy. An oligonucleotide or a pharmaceutically acceptable salt thereof, wherein the oligonucleotide comprises an oligonucleotide of 15-30 bases consisting of a nucleotide sequence complementary to the region of nucleotide Nos. 502-556 or 578-612 of DUX4-fl mRNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1; the 5? and/or 3? end of the oligonucleotide may be chemically modified; and the oligonucleotide is capable of switching the splice form of the DUX4 gene from DUX4-fl to DUX4-s. A pharmaceutical drug comprising the above oligonucleotide or a pharmaceutically acceptable salt thereof (e.g. therapeutic for facioscapulohumeral muscular dystrophy).
    Type: Application
    Filed: July 10, 2020
    Publication date: November 17, 2022
    Applicants: DAIICHI SANKYO COMPANY, LIMITED, Tokai University Educational System
    Inventors: Makoto KOIZUMI, Akifumi NAKAMURA, Takahiro KATAGIRI, Hiroaki MITSUHASHI
  • Publication number: 20220296647
    Abstract: Provided is reproducible means that enables production of nucleus pulposus progenitor cells (preferably, an active nucleus pulposus progenitor cell phenotype) from desired cells such as terminally differentiated cells and stem cells having pluripotency or multipotency. A nucleus pulposus progenitor cell inducer according to the present invention comprising an effective amount of a gene of Brachyury (T) or a homolog thereof, at least one selected from the group consisting of SRY-box6 (SOX6) or a homolog thereof and Forkhead Box Q1 (FOXQ1) or a homolog thereof, and MYC Proto-Oncogene, BHLH Transcription Factor (cMyc) or a homolog thereof (nucleus pulposus progenitor cell master regulator transcription factor), or a product thereof.
    Type: Application
    Filed: July 29, 2021
    Publication date: September 22, 2022
    Applicant: Tokai University Educational System
    Inventors: Daisuke SAKAI, Jordy SCHOL, Yoshihiko NAKAMURA
  • Publication number: 20220257833
    Abstract: Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.
    Type: Application
    Filed: April 26, 2022
    Publication date: August 18, 2022
    Applicants: CellSeed Inc., TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Masato SATO, Chikako SATO, Tomomi KOTOKU, Tomoko KAWAKE
  • Publication number: 20220184138
    Abstract: Provided is reproducible means that enables the production of an active nucleus pulposus cell phenotype from desired cells such as terminally differentiated cells or pluripotent or multipotent stem cells. Provided is a differentiation inducer containing an effective amount of a gene of at least two transcription factors selected from the group consisting of Brachyury (T), SRY-box6 (SOX6), C and Forkhead Box Q1 (FOXQ1), or homologs thereof (nucleus pulposus cell master regulator transcription factor), or a product thereof.
    Type: Application
    Filed: February 6, 2020
    Publication date: June 16, 2022
    Applicants: Tokai University Educational System, Kyoto University
    Inventors: Daisuke SAKAI, Jordy SCHOL, Shunsuke HIRAISHI, Shinji MASUI
  • Publication number: 20220115168
    Abstract: The present invention provides: a compound superconducting twisted wire in which non-adhesiveness between compound superconducting strands or separation easiness after adhesion is improved while a strength against tension is improved to a degree to be equivalent to or stronger than that of a conventional compound superconducting twisted wire; and a rewinding method thereof.
    Type: Application
    Filed: September 20, 2019
    Publication date: April 14, 2022
    Applicants: FURUKAWA ELECTRIC CO., LTD., TOHOKU UNIVERSITY, TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Masahiro SUGIMOTO, Hirokazu TSUBOUCHI, Daisuke ASAMI, Hideki II, Satoshi AWAJI, Hidetoshi OGURO
  • Publication number: 20220079995
    Abstract: An object of the present invention is to provide an agent for promoting expression of N-acetylgalactosaminyltransferase that contains an extract from inflamed tissues inoculated with vaccinia virus. The present invention demonstrated that the extract from inflamed tissues inoculated with vaccinia virus promotes the expression of N-acetylgalactosaminyltransferase in intervertebral disc cells. Thus, the extract from inflamed tissues inoculated with vaccinia virus or a preparation containing the extract is useful as an agent for promoting the expression of N-acetylgalactosaminyltransferase in intervertebral disc cells.
    Type: Application
    Filed: November 26, 2021
    Publication date: March 17, 2022
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, NIPPON ZOKI PHARMACEUTICAL CO., LTD.
    Inventors: Daisuke SAKAI, Tomoko NAKAI, Mitsuru NAIKI, Yoji SHIBAYAMA
  • Publication number: 20220064592
    Abstract: Cell microsheets are formed from a culture of cells. The cell microsheets has a size that can pass through an injection needle with a certain thickness. The cell microsheets can be produced on a surface of a cell cultureware. A stimulus-responsive polymer is immobilized on the surface having small divisions of the cell cultureware. The cell microsheets are suitable for minimally invasive treatment.
    Type: Application
    Filed: September 2, 2021
    Publication date: March 3, 2022
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, CellSeed Inc.
    Inventors: Masato SATO, Shiho WASAI, Yuka KAWAGUCHI
  • Publication number: 20220056418
    Abstract: Preparing a cell population rich in cells having a given phenotype depending on their use (e.g., type II collagen-positive nucleus pulposus cells) from a cell population containing Tie2-positive stem/progenitor cells (e.g., nucleus pulposus stem/progenitor cells). The present invention provides culture methods wherein a cell population containing Tie2-positive stem/progenitor cells is cultured (1) while present in a non-digested tissue, (2) in a culture medium containing at least one kind of Tie2 expression enhancer other than growth factors, (3) using cultureware with a culture surface having undergone cell attachment-increasing treatment, or (4) while suppressing formation of spheroid colonies in a culture medium containing an extracellular matrix-degrading agent.
    Type: Application
    Filed: March 25, 2020
    Publication date: February 24, 2022
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, NIPPON ZOKI PHARMACEUTICAL CO., LTD.
    Inventors: Daisuke SAKAI, Yoshihiko NAKAMURA, Erika MATSUSHITA
  • Publication number: 20210128699
    Abstract: Provided are a neutrophil activation regulator and a therapeutic agent against diseases caused by neutrophil activation. A thrombin-like enzyme is used as an active ingredient of the neutrophil activation regulator and the therapeutic agent against diseases caused by neutrophil activation.
    Type: Application
    Filed: January 12, 2018
    Publication date: May 6, 2021
    Applicants: TOBISHI PHARMACEUTICAL CO., LTD., TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Haruchika MASUDA, Takayuki ASAHARA, Hirobumi SENGA
  • Publication number: 20200392223
    Abstract: A low-molecular-weight compound (IL-17 activity inhibitor) having an IL-17 activity-inhibiting ability.
    Type: Application
    Filed: February 22, 2019
    Publication date: December 17, 2020
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, NIPPON ZOKI PHARMACEUTICAL CO., LTD.
    Inventors: Daisuke SAKAI, Noriaki HIRAYAMA, Kaori SUYAMA
  • Publication number: 20200384044
    Abstract: Provided is a method of detecting presence or absence of a functional gastrointestinal disorder by measuring bacteria occupancy within a human stomach.
    Type: Application
    Filed: December 26, 2017
    Publication date: December 10, 2020
    Applicants: MEIJI CO., LTD., TOKAI UNIVERSITY EDUCATIONAL SYSTEM
    Inventors: Yasuhiro Koga, Hirohiko Nakae, Toshihiro Ohtsu
  • Publication number: 20200386765
    Abstract: An objective is to provide a marker that enables a diabetic complication to be examined. There is provided the marker for examining a diabetic complication, comprising a compound represented by the following Formula (1), or a salt thereof.
    Type: Application
    Filed: November 16, 2018
    Publication date: December 10, 2020
    Applicants: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, RIKEN
    Inventors: Ryoji NAGAI, Naoyuki TANIGUCHI
  • Patent number: 10668281
    Abstract: In order to make advanced gait training easy and effective, an electrical spinal cord stimulator for gait training used in gait training for those who have difficulty in walking due to hemiplegia includes: first and second electrodes attached to a rehabilitant; a detector which detects a gait-related physical movement of the rehabilitant; and an electrical sensory nerve stimulation generator which generates, according to a detection result of the detector, electrical stimulation to be applied through the first and second electrodes to a nerve root of a sensory nerve which communicates with a spinal cord.
    Type: Grant
    Filed: March 14, 2016
    Date of Patent: June 2, 2020
    Assignees: Tokai University Educational System, Keio University
    Inventors: Toshiyuki Fujiwara, Yoshihisa Masakado, Junichi Ushiba
  • Patent number: 10533158
    Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.
    Type: Grant
    Filed: February 28, 2006
    Date of Patent: January 14, 2020
    Assignees: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, CELLSEED, INC.
    Inventors: Masato Sato, Hideaki Sakai