Patents Assigned to Touchlight IP Limited
-
Publication number: 20230272461Abstract: The present invention relates to improved processes for production of closed linear deoxyribonucleic acid (DNA), in particular cell-free enzymatic production of closed linear DNA molecules, preferable using a closed linear DNA as a template for DNA synthesis. The invention further relates to a novel closed linear DNA species, suitable for use as a template in the improved processes for production of closed linear DNA. Further, the invention pertains to the intermediate products of the processes, since this enables the production of larger quantities of closed linear DNA from the template than with methods known in the art.Type: ApplicationFiled: October 6, 2022Publication date: August 31, 2023Applicant: Touchlight IP LimitedInventors: Thomas ADIE, Neil PORTER, Paul ROTHWELL
-
Publication number: 20230091493Abstract: The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale, with an improved yield and/or with an improved efficiency. The invention requires the use of nucleotide complexes wherein the nucleotide is associated with a mixture of divalent and monovalent cations. Preferably, the divalent cation may be magnesium or manganese.Type: ApplicationFiled: February 15, 2021Publication date: March 23, 2023Applicants: Touchlight IP Limited, Touchlight IP LimitedInventors: Paul ROTHWELL, Daniel KYSH, Neil PORTER
-
Patent number: 11505821Abstract: The present invention relates to improved processes for production of closed linear deoxyribonucleic acid (DNA), in particular cell-free enzymatic production of closed linear DNA molecules, preferably using a closed linear DNA as a template for DNA synthesis. The invention further relates to a novel closed linear DNA species, suitable for use as a template in the improved processes for production of closed linear DNA. Further, the invention pertains to the intermediate products of the processes, since this enables the production of larger quantities of closed linear DNA from the template than with methods known in the art.Type: GrantFiled: August 16, 2017Date of Patent: November 22, 2022Assignee: Touchlight IP LimitedInventors: Thomas Adie, Neil Porter, Paul Rothwell
-
Publication number: 20220127667Abstract: The present invention relates to an in vitro cell-free process for production of deoxyribonucleotides (DNAs) comprising at least one hairpin, corresponding DNA products and uses thereof, and oligonucleotides and kits useful in the process of the invention.Type: ApplicationFiled: September 15, 2021Publication date: April 28, 2022Applicant: Touchlight IP LimitedInventors: Paul ROTHWELL, Neil PORTER, Lydia VINEY, Thomas ADIE
-
Patent number: 11149302Abstract: The present invention relates to an in vitro cell-free process for production of deoxyribonucleotides (DNAs) comprising at least one hairpin, corresponding DNA products and uses thereof, and oligonucleotides and kits useful in the process of the invention.Type: GrantFiled: February 17, 2016Date of Patent: October 19, 2021Assignee: Touchlight IP LimitedInventors: Paul Rothwell, Neil Porter, Lydia Viney, Thomas Adie
-
Patent number: 10501782Abstract: The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.Type: GrantFiled: August 21, 2015Date of Patent: December 10, 2019Assignee: Touchlight IP LimitedInventors: Neil Porter, Paul Rothwell, Jonathan Extance
-
Publication number: 20190185924Abstract: The present invention relates to improved processes for production of closed linear deoxyribonucleic acid (DNA), in particular cell-free enzymatic production of closed linear DNA molecules, preferably using a closed linear DNA as a template for DNA synthesis. The invention further relates to a novel closed linear DNA species, suitable for use as a template in the improved processes for production of closed linear DNA. Further, the invention pertains to the intermediate products of the processes, since this enables the production of larger quantities of closed linear DNA from the template than with methods known in the art.Type: ApplicationFiled: August 16, 2017Publication date: June 20, 2019Applicant: Touchlight IP LimitedInventors: Thomas ADIE, Neil PORTER, Paul ROTHWELL
-
Patent number: 10286399Abstract: Apparatus for biochemical synthesis having a reaction vessel, a temperature control device for the reaction vessel, a plurality of reservoirs for reaction components, and supply/withdrawal systems, e.g. reciprocating syringe pumps and switchable valves. Agitation of a reaction mixture is effected by withdrawing a part of the mixture from the reaction vessel and returning it thereto.Type: GrantFiled: March 4, 2014Date of Patent: May 14, 2019Assignee: Touchlight IP LimitedInventors: Neil Porter, Paul James Rothwell
-
Patent number: 9499847Abstract: A primer for the amplification of a DNA template comprising a protelomerase target sequence, particularly for production of closed linear DNA, which primer is capable of specifically binding to a palindromic sequence within a protelomerase target sequence and priming amplification in both directions.Type: GrantFiled: August 4, 2011Date of Patent: November 22, 2016Assignee: Touchlight IP LimitedInventors: Neil Porter, Lisa Caproni, Karen Oliver, Kinga Karbowniczek, Angus Knight