Abstract: A method for transforming algal or cyanobacterial cells, comprising mixing a polynucleotide for transforming the cells with the polynucleotide; performing microporation by applying a plurality of electrical pulses to the cells with a microporation apparatus; and incubating said polynucleotide with the cells after said applying said electrical pulses.
Abstract: This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells.
Abstract: The high-speed centrifugation heretofore required for harvesting micro algae and cyanobacteria cultured for biofuels and other co-products is a major cost constraint. Mixing algae/cyanobacteria at high-density culture with far less alkali than previously assumed is sufficient to flocculate the cells. The amount of flocculant required is a function of the logarithm of cell density, and is not a linear function of cell density as had been thought. The least expensive alkali treatments are with slaked limestone or dolomite (calcium hydroxide and magnesium hydroxides). Further water can be removed from the floc by sedimentation, low speed centrifugation, dissolved air flotation or filtration, prior to further processing to separate oil from valuable co-products.
Type:
Application
Filed:
September 28, 2010
Publication date:
April 7, 2011
Applicant:
TransAlgae Ltd
Inventors:
Ami Schlesinger, Doron Eisenstadt, Shai Einbinder, Jonathan Gressel
Abstract: This disclosure provides a method to reduce cell damage caused by near UV light absorption of algal or cyanobacterial cultures. The algal or cyanobacterial cells are transformed to express one or more fluorescent proteins, that absorb the harmful UV or near UV wavelengths and emits wavelengths that are photosynthetically more active. The photosynthetic pigments of the transgenic algal or cyanobacterial cell culture will then absorb the photosynthetically active light emitted by the fluorescent proteins. Accordingly the harmful effects of the UV and near UV radiation are reduced and the photosynthetic activity of the algal or cyanobacterial cells is improved.
Abstract: Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO2 emissions back to products, and so as not to be able to grow outside of cultivation.
Type:
Application
Filed:
September 8, 2009
Publication date:
April 1, 2010
Applicant:
TransAlgae Ltd
Inventors:
Daniella Schatz, Jonathan Gressel, Doron Eisenstadt, Shai Ufaz
Abstract: This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells.
Abstract: Virus contaminations of bioreactors can cause considerable losses in the industry and prevention of such contaminations is usually a major concern, especially in continuous cultures and particularly in outdoor/uncovered operations such as ponds or “racing ponds”. Use of transgenic algae/cyanobacteria harboring introgressed virus/phage DNA fragments, cultured in these bioreactors/ponds will provide protection to a range of viruses/phages. Molecular mechanisms such as lysogeny and post transcriptional gene silencing (PTGS) are being exploited to produce protected algae/cyanobacteria with cross protective resistance against various viruses/phage, thus gaining bioreactor stability.
Type:
Application
Filed:
September 8, 2009
Publication date:
March 18, 2010
Applicant:
TransAlgae Ltd
Inventors:
Jonathan Gressel, Ofra Chen, Michael Danon