Abstract: Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.

Abstract: Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.

Abstract: A process for the preparation of short-chain alkyl esters of fatty acids, preferably fatty acid methyl esters (biodiesel) in solvent-free system is disclosed. In particular, the process comprises (a) providing a fatty acid source, (b) stepwise adding a short-chain free alcohol, preferably methanol, or any other alcohol donor, to said fatty acid source in the presence of a lipase preparation and (c) allowing the reaction to proceed under suitable conditions, until said fatty acid source triglycerides are converted to fatty acid methyl esters (FAME). The lipase preparation comprises at least two lipases. The two lipases being separately or jointly immobilized on a suitable support and wherein at least one of the lipases has increased affinity for partial glycerides and at least one of the lipases is sn-1,3 positional specific.

Abstract: Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.

Abstract: A process for the preparation of short-chain alkyl esters of fatty acids, preferably fatty acid methyl esters (biodiesel) in solvent-free system is disclosed. In particular, the process comprises (a) providing a fatty acid source, (b) stepwise adding a short-chain free alcohol, preferably methanol, or any other alcohol donor, to said fatty acid source in the presence of a lipase preparation and (c) allowing the reaction to proceed under suitable conditions, until said fatty acid source triglycerides are converted to fatty acid methyl esters (FAME). The lipase preparation comprises at least two lipases. The two lipases being separately or jointly immobilized on a suitable support and wherein at least one of the lipases has increased affinity for partial glycerides and at least one of the lipases is sn-1,3 positional specific.