Abstract: A cellular microcompartment comprising: at least one layer of cells, an outer layer of hydrogel, and a fibrin mesh arranged between the outer layer of hydrogel and said layer of cells, the cellular microcompartment being compliant with regulations for Good Manufacturing Practices (GMP) and suitable for clinical applications, as well as methods for preparing the cellular microcompartment, and methods in which the cellular microcompartment is used for production of cells and/or tissues, including a large-scale production.

Abstract: The invention relates to cellular microcompartments, each microcompartment successively comprising the following layers, which are organised around at least one lumen: —at least one inner layer of human cells undergoing cardiac differentiation, expressing at least one gene chosen from PDGFR?, MESP-1, NKX2-5, GATA4, MEF2C, TBX20, ISL1 and TBX5, —at least one intermediate layer of isotonic aqueous solution, and—at least one outer hydrogel layer. The invention also relates to the cardiac tissues obtained from said microcompartments and the use thereof, particularly in the treatment of heart disease.