Patents Assigned to Trilink Biotechnologies
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Publication number: 20240158433Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: January 9, 2024Publication date: May 16, 2024Applicant: TriLink BioTechnologies, LLCInventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Publication number: 20240140982Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: September 14, 2023Publication date: May 2, 2024Applicant: TriLink Biotechnologies, LLCInventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Patent number: 11878991Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7 Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: November 6, 2020Date of Patent: January 23, 2024Assignee: TriLink BioTechnologies, LLCInventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Patent number: 11578095Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: May 26, 2022Date of Patent: February 14, 2023Assignee: TriLink Biotechnologies, LLCInventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Publication number: 20220289786Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: May 26, 2022Publication date: September 15, 2022Applicant: TriLink Biotechnologies, LLCInventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Patent number: 11414453Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: August 2, 2021Date of Patent: August 16, 2022Assignee: TriLink Biotechnologies, LLCInventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Publication number: 20210371452Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: August 2, 2021Publication date: December 2, 2021Applicant: TriLink Biotechnologies, LLCInventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Publication number: 20210261597Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7 Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: November 6, 2020Publication date: August 26, 2021Applicant: TriLink Biotechnologies, LLCInventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Patent number: 10913768Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: September 20, 2016Date of Patent: February 9, 2021Assignee: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Patent number: 10519189Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: December 26, 2018Date of Patent: December 31, 2019Assignee: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Patent number: 10494399Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: GrantFiled: April 1, 2019Date of Patent: December 3, 2019Assignee: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Publication number: 20190270766Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: April 1, 2019Publication date: September 5, 2019Applicant: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Publication number: 20190144490Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: December 26, 2018Publication date: May 16, 2019Applicant: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Publication number: 20180273576Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.Type: ApplicationFiled: September 20, 2016Publication date: September 27, 2018Applicant: TRILINK BIOTECHNOLOGIES, INC.Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Publication number: 20170275672Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.Type: ApplicationFiled: April 24, 2017Publication date: September 28, 2017Applicant: TriLink BioTechnologies, Inc.Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
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Patent number: 9631227Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.Type: GrantFiled: March 15, 2013Date of Patent: April 25, 2017Assignee: TRILINK BIOTECHNOLOGIES, INC.Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
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Publication number: 20140323354Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.Type: ApplicationFiled: March 15, 2013Publication date: October 30, 2014Applicant: Trilink BioTechnologies, Inc.Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
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Patent number: 8728725Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template.Type: GrantFiled: July 6, 2010Date of Patent: May 20, 2014Assignee: Trilink BiotechnologiesInventors: Natasha Paul, Jonathan Shum, Alexandre Lebedev, Gerald Zon
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Patent number: 8361753Abstract: The present invention provides methods and compositions for nucleic acid amplification. These methods involve the use of oligonucleotide primers in temperature dependent nucleic acid amplification reactions. In certain aspects, the methods are accomplished by use of certain modified oligonucleotide primers which provide utility in nucleic acid amplification. In preferred embodiments, the oligonucleotide primers are modified with particular chemical groups such as esters.Type: GrantFiled: May 17, 2007Date of Patent: January 29, 2013Assignee: TriLink BioTechnologies, Inc.Inventors: Gerald Zon, Alexandre Lebedev
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Patent number: 8133669Abstract: Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3?-substituted nucleoside 5?-triphosphates or 3?-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3?-substituted NTPs and/or 3?-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3?-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.Type: GrantFiled: May 21, 2009Date of Patent: March 13, 2012Assignee: Trilink BiotechnologiesInventors: Alexandre Lebedev, Inna Koukhareva