Abstract: Provided herein is a method for processing a liquid sample, utilizing a first component including an interface side and at least 3 sample containers or tanks; and a second component including a connector manifold. The method may include the step of reversibly connecting a reagent container, a filtration apparatus, and a chromatography apparatus to the manifold; reversibly connecting the first component to the connector manifold; pressurizing the first sample tank; transferring the sample into the filtration apparatus; passing the sample through a filter; transferring the sample into the second sample tank; transferring the sample into the chromatography apparatus; transferring the reagent into the chromatography apparatus; and transferring the sample to the third sample tank. Some or all transfers may be routed via the connector manifold and the interface side.

Abstract: Provided herein is a method for sterile fluid transfer. A first array of conduits may be disposed in a first compartment having a planar face with an array of cavities, each containing a cannular protrusion fluidly connected to a conduit. A second array of conduits may be disposed in a second compartment having its own planar face with an array of surface cannular protrusions, each fluidly connected to a conduit in the second array. The method may include sterilizing the lumens of each conduit-protrusion pair; snugly juxtaposing the two planar faces to form an array of steam-tight chambers; introducing a sterilizing gas into the chambers; expelling the sterilizing gas from the chambers; moving each cannular protrusion towards its facing surface protrusion to reversibly form a fluid connection therebetween, while the chamber remains externally sealed, and transferring a fluid across at least one fluidic connection.

Abstract: Provided herein is a method for incubating living cells, in accordance with principles of the disclosure, may include the steps of: (a) providing a pressurized gas to a medium reservoir, the pressurized gas providing an impetus that moves a growth medium in the medium reservoir to a bioreactor chamber via a first incoming fluid line connecting the medium reservoir to the bioreactor chamber; (b) simultaneously or subsequently to step (a), providing a pressurized gas to a cell reservoir holding a suspension of the living cells, the pressurized gas providing an impetus that moves the suspension to the bioreactor chamber via a second incoming fluid line connecting the cell reservoir to the bioreactor chamber; and (c) incubating the growth medium and the living cells in the bioreactor chamber, under conditions compatible with cell viability.