Patents Assigned to Trustees of Boston University
  • Publication number: 20180163195
    Abstract: The technology described herein relates to controlled chemically- or light-induced rejoinder of split-recombinases. In some embodiments, compositions, methods, kits and systems are provided that relate to a split-recombinase system, whereby protein complementation or rejoinder of split-recombinases is mediated by chemical-induced dimerization domains (CIDDs) or light-induced dimerization domains (LIDD) and rejoinder of the split-recombinases occur in the presence of one or more chemical inducers or light inducers, respectively. The split-recombinases systems as disclosed herein can be used in gene therapy, integrated logic and memory in living cells such as mammalian cells. The nucleic acid cassettes, switches, and systems described herein allow for controlled gene expression or gene regulation. The controlled chemically- or light-induced rejoinder of split-recombinases can be used in, for example, adopted T-cell therapy.
    Type: Application
    Filed: December 12, 2017
    Publication date: June 14, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Wilson W. WONG, Benjamin Harris WEINBERG
  • Patent number: 9993577
    Abstract: The inventions provided herein relate to dissolvable hydrogel compositions and methods of uses, e.g., but not limited to, in wound management. Accordingly, methods for wound management involving the dissolvable hydrogel compositions are also provided herein. In some embodiments, the dissolvable hydrogel composition comprises an adhesive thioester hydrogel, which can facilitate adherence of the dissolvable hydrogen composition to a surface (e.g., a wound) and can be controllably dissolved later upon addition of a thiolate compound to release the dissolvable hydrogel composition from the surface (e.g., the wound).
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: June 12, 2018
    Assignee: Trustees of Boston University
    Inventors: Mark W. Grinstaff, Cynthia Ghobril, Michel Christophe Wathier, Marlena Dagmara Konieczynska
  • Publication number: 20180155758
    Abstract: A system and method for antibiotic susceptibility testing efficiently determines whether bacteria are alive or have been killed by antibiotic treatment. The antibiotic susceptibility testing device includes at least one reservoir into which a bacteria solution is introduced and a microfluidic channel connected to the reservoir, wherein the cross-sectional size of the microfluidic channel is selected to be comparable to the size of the bacterium to be tested. Furthermore, the electrical resistance or voltage signal across the microchannel is monitored as bacteria swim into and out of the channel. Alternatively, a small population of bacteria can be immobilized in the microchannel. The resistance or voltage signal fluctuates when the bacteria are alive and moving in and out of the channel or wiggling on the microchannel walls. If the bacteria are dead, they have limited motility and the signal fluctuations are significantly smaller.
    Type: Application
    Filed: February 2, 2018
    Publication date: June 7, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Kamil Ekinci, Le Li, Chuanhua Duan, Vural Kara, Deborah Stearns-Kurosawa
  • Publication number: 20180142247
    Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5? untranslated region (5? UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5? UTR of an mRNA.
    Type: Application
    Filed: November 30, 2016
    Publication date: May 24, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: James J. Collins, Farren J. Isaacs, Charles R. Cantor, Daniel J. Dwyer
  • Patent number: 9979038
    Abstract: System and method for energy storage and recovery is described. More particularly, system and method using tungsten based materials to electrochemically store and recover energy is described. In certain embodiments, the system includes a reversible solid oxide electrochemical cell (RSOEC) having a porous cathode, a porous anode, and an electrolyte capable of transporting oxygen ion. The system further includes a reactor comprising tungsten, tungsten oxide, or combinations thereof. To store the energy, the RSOEC is capable of receiving electricity to electrolyze H2O to generate H2 and O2 and the reactor is operably connected to the RSOEC to receive the generated H2 and convert tungsten oxide to tungsten thereby storing electrical energy. To recover the energy, reactor is capable of receiving H2O to convert tungsten to tungsten oxide and generate H2 and the RSOEC is operably connected to the reactor to receive the generated H2 and generate electrical energy.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: May 22, 2018
    Assignee: Trustees of Boston University
    Inventors: Uday B. Pal, Srikanth Gopalan
  • Patent number: 9957511
    Abstract: Various aspects and embodiments of the present disclosure are directed to methods and compositions for functionalizing endogenous bacteria in vivo. The methods include delivering to endogenous bacterial cells a recombinant bacteriophage or phagemid that is engineered to contain at least one genetic circuit.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: May 1, 2018
    Assignees: Massachusetts Institute of Technology, Trustees of Boston University
    Inventors: Timothy Kuan-Ta Lu, Robert James Citorik, James Collins, Russell-John Krom
  • Publication number: 20180110404
    Abstract: Disclosed herein is an aspiration assembly for attaching to an endoscope. The aspiration assembly comprises an extendable suction tube for aspirating an object (e.g., smoke, blood, blood clot, bone debris, or tissue debris) at or near a surgical site during an endoscopic surgery. The aspiration assembly can further comprise an irrigation tube for directing a fluid to the surgical site for cleaning the endoscope lens. By integrating an extendable suction tube with the endoscope, the technology described herein obviate the need for instrument switching and/or multiple surgeons during the surgery.
    Type: Application
    Filed: April 15, 2016
    Publication date: April 26, 2018
    Applicants: TRUSTEES OF BOSTON UNIVERSITY, BOSTON MEDICAL CENTER CORPORATION, FRAUNHOFER USA, INC.
    Inventors: Anand DEVAIAH, Andre SHARON, Alexis SAUER-BUDGE, Holger WIRZ, Yuzhang YANG, Stephanus Johannes Marcellis VAN DER KEMP, Daniel ECHEVERRIA
  • Publication number: 20180099072
    Abstract: The present invention provides polymers which can be used as tissue supplements or lubricants in vivo. The inventive polymers can be used as tissue-interpenetrating hydrogel supplements, viscosupplements, tribosupplements, viscoelastics, tissue space fillers, and/or anti-adhesive agents. Also provided are pharmaceutical compositions comprising the inventive polymers and methods of using them including, for example, in the treatment of arthritic and injured synovial joints; in reconstruction or cosmetic procedures, intervertebral disc repair, treatment of vocal cord problems, treatment of urinary incontinence, and prevention of adhesion formation following abdominal or gynecological surgery or malfunction of naturally lubricious mucosal tissue.
    Type: Application
    Filed: December 13, 2017
    Publication date: April 12, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Mark GRINSTAFF, Benjamin Goldman COOPER
  • Patent number: 9927350
    Abstract: The present invention relates to frequency domain thermoreflectance (FDTR) imaging of a thermophysical property or a set of thermophysical properties of a sample. A method comprises measuring the amplitude and/or phase of a beam of radiation reflected from a sample surface, while a heat source applied to the sample is modulated at at least two modulation frequencies simultaneously. Such measurement can be reiterated as a probe beam is scanned across the sample surface or a portion thereof. A 2D image or map of a thermophysical property or a set of thermophysical properties can be generated from data processing. Also provided herein is an apparatus for performing FDTR imaging.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: March 27, 2018
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventor: Aaron J. Schmidt
  • Patent number: 9920374
    Abstract: The present invention provides methods for diagnosis and prognosis of lung cancer using expression analysis of one or more groups of genes, and a combination of expression analysis with bronchoscopy. The methods of the invention provide far superior detection accuracy for lung cancer when compared to any other currently available method for lung cancer diagnostic or prognosis. The invention also provides methods of diagnosis and prognosis of other lung diseases, particularly in individuals who are exposed to air pollutants, such as cigarette or cigar smoke, smog, asbestos and the like air contaminants or pollutants.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: March 20, 2018
    Assignee: Trustees of Boston University
    Inventors: Jerome S. Brody, Avrum Spira, Jennifer E. Beane-Ebel, Marc E. Lenburg
  • Patent number: 9919009
    Abstract: This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a stem cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR agonist are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs.
    Type: Grant
    Filed: June 2, 2015
    Date of Patent: March 20, 2018
    Assignees: BOSTON MEDICAL CENTER CORPORATION, TRUSTEES OF BOSTON UNIVERSITY
    Inventors: George J. Murphy, David H. Sherr, Sarah S. Rozelle, Brenden W. Smith
  • Patent number: 9913862
    Abstract: Provided herein are methods and compositions for treatment of a microbial infection or a microbial biofilm in a subject or on a surface by administering to the subject or surface determined to have or suspected of having a microbial infection/film an antimicrobial agent in combination with a silver-containing compound (e.g., a silver salt). In some embodiments, a silver-containing compound can increase activity of the antimicrobial agent. In other embodiments, addition of a silver-containing compound to an antimicrobial agent can expand the antimicrobial spectrum of the antimicrobial agent such that the antimicrobial agent originally indicated for treatment of one microbial strain (e.g., Gram-positive microbes) becomes effective for treating additional microbial strains (e.g., Gram-negative microbes). Other aspects relating to methods and compositions for delivering an agent to a microbe by increasing the membrane permeability of the microbe are also provided herein.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: March 13, 2018
    Assignee: Trustees of Boston University
    Inventors: James J. Collins, J. Ruben Morones-Ramirez, Jonathan Alexander Winkler, Catherine S. Spina
  • Publication number: 20180057838
    Abstract: Embodiments disclosed herein provide artificial expression systems comprising the zinc-finger containing transcription factors and engineered promoters to modulate expression of genes of interest. Engineered zinc-finger transcription factors that interact with engineered promoters constitute synthetic and regulatable expression systems which facilitate the modulation of gene expression as desired.
    Type: Application
    Filed: August 25, 2017
    Publication date: March 1, 2018
    Applicants: TRUSTEES OF BOSTON UNIVERSITY, THE GENERAL HOSPITAL CORPORATION
    Inventors: Ahmad S. Khalil, Divya Israni, Minhee Park, J. Keith Joung, Jeffry D. Sander
  • Patent number: 9903820
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproducibly ceased, and (2) self-assembly under voltage driven electrolyte flow, in which 5 nm nanopore may be coated. Applications of chemically modified nanopore are provided including, the detection of biopolymers such as DNA and RNA, immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: February 27, 2018
    Assignee: The Trustees of Boston University
    Inventors: Amit Meller, Meni Wanunu
  • Publication number: 20180051277
    Abstract: Described herein are approaches for the improved detection, identification, and/or quantification of target nucleic acids. These approaches provide a means of detecting, identifying, and/or quantifying rare target nucleic acid molecules, including DNA and RNA molecules, from the same sample, and in the same reaction, by using “hairpin barcode primers,” as the term is defined herein, to incorporate unique barcodes into target nucleic acids in a PCR pre-amplification step.
    Type: Application
    Filed: February 24, 2016
    Publication date: February 22, 2018
    Applicants: Trustees of Boston University, Ontario Institute for Cancer Research Mars Centre
    Inventors: Tony Edward GODFREY, Anders Torbjoern STAAHLBERG, Paul KRZYZANOWSKI
  • Publication number: 20180051311
    Abstract: A system and method for antibiotic susceptibility testing efficiently determines whether bacteria are alive or have been killed by antibiotic treatment. The antibiotic susceptibility testing device includes at least one reservoir into which a bacteria solution is introduced and a microfluidic channel connected to the reservoir, wherein the cross-sectional size of the microfluidic channel is selected to be comparable to the size of the bacterium to be tested. Furthermore, the electrical resistance or voltage signal across the microchannel is monitored as bacteria swim into and out of the channel. Alternatively, a small population of bacteria can be immobilized in the microchannel. The resistance or voltage signal fluctuates when the bacteria are alive and moving in and out of the channel or wiggling on the microchannel walls. If the bacteria are dead, they have limited motility and the signal fluctuations are significantly smaller.
    Type: Application
    Filed: August 4, 2017
    Publication date: February 22, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Kamil Ekinci, Le Li, Chuanhua Duan, Vural Kara, Deborah Stearns-Kurosawa
  • Publication number: 20180051033
    Abstract: The present invention is directed to methods, compositions and kits for treatment of cancer, e.g. heptacellular carcinoma. In some embodiments, the present invention discloses the use of a small-molecule compound of formula (I)-(XXVI) and (III?) as disclosed herein to inhibit transcription factor Late SV40 Factor (LSF) for treatment of cancer, e.g., HCC.
    Type: Application
    Filed: September 25, 2017
    Publication date: February 22, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Ulla Hansen, Scott Schaus, Trevor Grant, Joshua Bishop, John Kavouris, Lisa M. Christadore
  • Patent number: 9896660
    Abstract: This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a MEP precursor cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) modulator. In some embodiments the AhR modulator is an AhR antagonist. In some embodiments the AhR modulator is an AhR agonist. In some embodiments the methods comprise culturing MEP precursor cells in the presence of an AHR antagonist and then culturing MEP precursor cells in the presence of an AHR agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR modulator are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: February 20, 2018
    Assignees: BOSTON MEDICAL CENTER CORPORATION, TRUSTEES OF BOSTON UNIVERSITY
    Inventors: George J. Murphy, David H. Sherr, Sarah S. Rozelle, Brenden W. Smith
  • Publication number: 20180043066
    Abstract: Provided herein are methods comprising the use of a macroinitiator and application protocols to apply a hydrophilic coating to latex, or natural rubber, and compositions resulting from such methods. This coating results in e.g., an increased sense of lubrication when in contact with water or an aqueous solution.
    Type: Application
    Filed: February 22, 2016
    Publication date: February 15, 2018
    Applicants: TRUSTEES OF BOSTON UNIVERSITY, BOSTON MEDICAL CENTER CORPORATION
    Inventors: Mark W. Grinstaff, Stacy L. Chin, Ruiqing Xiao, Benjamin Goldman Cooper, Karen Buch, Ducksoo Kim
  • Publication number: 20180028683
    Abstract: Provided herein are theranostic compositions comprising a Janus nanoparticle-coated microbubble that are useful for imaging (e.g., MRI, or ultrasound) and for delivering a therapeutic or bioactive agent (e.g., nucleic acid(s), drugs, etc), among other uses.
    Type: Application
    Filed: February 18, 2016
    Publication date: February 1, 2018
    Applicant: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Joyce Y. Wong, Ragnhild D. Whitaker, Nelson Ruiz-Opazo, Victoria L.M. Herrera