Abstract: Compositions and methods for regulating CD154 gene expression are provided that rely on the interaction of polypyrimidine tract proteins with the 3?-untranslated region of CD154.
Abstract: A first electrode is separated from a second electrode by an interelectrode space. The interelectrode space does not exceed 3 mm, and preferably does not exceed 100 ?m. Liquid water fills the interelectrode space, thereby electrically connecting the first electrode and the second electrode. A power supply, preferably low-frequency AC, is connected to the first and second electrodes, generating a current through the water in the interelectrode space. The applied electric power prevents freezing of a thin liquid water layer in the interelectrode space, thereby preventing ice formation.
Abstract: The present invention provides applicators for performing thermokeratoplasty. One embodiment of such applicators includes two generally tubular electrical conducting elements coaxially disposed relative to one another and separated by an electrically insulating element having a selected thickness. At least a portion of each conducting tube is coated with an electrically insulating and thermally conductive material, e.g., anodized aluminum, so as to electrically insulate the conducting tubes from corneal tissue upon placement of the applicator on a subject's cornea while facilitating extraction of heat from the cornea.
Abstract: Novel molecules of the multi-drug and toxin efflux (MATE) family of molecules, designated herein as mutant ferric reductase defective (FRD3) nucleic acid and protein molecules are disclosed. The FRD3 nucleic acid and protein molecules are useful as modulating agents in regulating metal homeostasis, e.g., iron homeostasis. The invention further describes transgenic plants in which expression of a FRD3 polypeptide of the invention is altered. Compositions containing FRD3 molecules and methods of using such molecules are also provided.
Abstract: The present invention relates to a method of detecting intracellular cholesterol. The method provides contacting a permeabilized cell with labeled C? complex. Methods of using this detection method to identify agents which modulate cholesterol accumulation in a cell are also provided.
Abstract: Novel tricyclic-bis-enone derivatives (TBEs) as well as the process for the preparation of such TBEs are provided. Also provided are methods for prevention and/or treatment of cancer, Alzheimer's disease, Parkinson's disease, multiple sclerosis, amyotropic lateral sclerosis, rheumatoid arthritis, inflammatory bowel disease, and all other diseases whose pathogenesis is believed to involve excessive production of either nitric oxide (NO) or prostaglandins or the overexpression of iNOS or COX-2 genes or gene products. Further, methods for the synthesis of the TBE compounds of the invention utilize cheap commercially available reagents and are highly cost effective and amenable to scale-up. Additional high efficiency synthetic methods that utilize novel intermediates as well as the synthesis of these intermediates are also provided. Furthermore, the invention also provides methods for designing novel and water-soluble TBEs.
Type:
Grant
Filed:
January 15, 2003
Date of Patent:
February 13, 2007
Assignee:
The Trustees of Dartmouth College
Inventors:
Tadashi Honda, Frank G. Favaloro, Gordon W. Gribble, Michael B. Sporn, Nanjoo Suh
Abstract: An animal selected for lacking heparan sulfate 3-O-sulfotransferase-1 activity is provided. This animal exhibits characteristics associated with myxomatous valvular disease and is useful for identifying agents which prevent, delay or treat myxomatous valvular disease. Methods of diagnosing myxomatous valvular disease are also provided.
Type:
Grant
Filed:
February 10, 2003
Date of Patent:
January 23, 2007
Assignees:
Trustees of Dartmouth College, Massachusetts Institute of Technology (MIT)
Inventors:
Nicholas W. Shworak, Robert D. Rosenberg, Robert T. Palac
Abstract: High-frequency AC voltage with a frequency in a range of from 60 to 100 kHz supplied from a power source at 3 to 15 kV is applied to an electrical conductor within about 30 cm of a cableway. The high-frequency AC voltage generates an alternating electric field. Capacitive AC current associated with the alternating electric field flows through the ice on the cableway and on the electrical conductor, causing dielectric loss heat that melts the ice.
Type:
Grant
Filed:
January 24, 2002
Date of Patent:
January 16, 2007
Assignee:
The Trustees of Dartmouth College
Inventors:
Victor F. Petrenko, Charles R. Sullivan
Abstract: The invention provides a drug combination for the treatment of diseases associated with bone metastasis. The combination provides adenosine or a derivative thereof, a bisphosphonate, and a targeting agent(s) to decrease the signs or symptoms associated with such diseases.
Abstract: A nucleic acid sequence required for regulating the autolytic activity of bacteria is provided. Also provided are polypeptides encoded by the gene or mutant gene as well as vector and host cells for expressing these polypeptides. Methods for identifying and using agents which interact with the gene or mutant gene or polypeptides encoded thereby to inhibit bacterial growth and infectivity are also provided.
Abstract: Methods for detecting the inflammatory biomarkers molecule CD 163 in biological samples are provided. Also provided are methods for monitoring the course of an inflammatory process or condition in a patient and compositions and methods for preventing and treating inflammation and inflammatory processes.
Type:
Grant
Filed:
March 27, 2001
Date of Patent:
December 5, 2006
Assignee:
Trustees of Dartmouth College
Inventors:
Paul M. Guyre, Peter M. Morganelli, Nicholas J. Goulding
Abstract: An electrical conductor and a gas-filled layer are located at or near the surface of an object being deiced. The conductor carries an AC voltage that generates an alternating electric field in the gas-filled layer. A conductive layer increases the electric field strength in the gas-filled layer between the electrical conductor and the conductive layer. The alternating electric field causes electric breakdown of gas and plasma-formation in the gas-filled layer. The plasma absorbs energy released during electric discharge through the plasma, which heats ice, causing it to melt. The alternating electric field typically has a field strength in a range of about from 1 to 100 kV/cm. The AC voltage typically has an amplitude in a range of about from 10 kV to 1300 kV, and a frequency in a range of about from 50 Hz to 1 MHz. The gas-filled layer includes a plasma-forming gas selected from, among others, air, nitrogen and argon.
Abstract: The infection of a mammalian host by a microorganism can be prevented or treated through the administration of substrates for transglutaminases or antibodies against such substrates that inhibit the transglutaminase-mediated interaction of the microorganism with the mammalian host. These compounds may be used in the identification, prevention or treatment of microbial infection of mammalian hosts such as immunocompromised or immunosuppressed humans, for example, those having AIDS or undergoing transplantation or anti-cancer therapy.
Abstract: An improved method of producing 3-amino-1,2,4-benzotriazines using nitrobenzene or a derivative thereof, a guanidine salt and a base as reactants is provided. The method is carried out at a moderate reaction temperature without producing halide wastes derived from nucleophilic substitution and acid byproducts.
Abstract: Disclosed are various genes encoding proteins that are shown to play a role, direct or indirect, in microbial resistance of an organism in a biofilm and homologs thereof. Also disclosed are methods of identifying a compound that modulates microbial resistance of an organism in a biofilm, and methods of identifying genes that encode proteins that play a role, direct or indirect, in biofilm resistance.
Abstract: A continuous environmental noise level recording and analysis system is provided. The system is capable of sampling, processing and storing Equivalent Sound Level values over a period of about two weeks. The recorded data is downloaded and analyzed to show graphs, and to automatically detect noise events of interest.
Type:
Grant
Filed:
October 25, 2002
Date of Patent:
August 15, 2006
Assignee:
The Trustees of Dartmouth College
Inventors:
Robert D. Collier, Douglas A. Fraser, Kenneth Kaliski, G. Ayorkor Mills-Tettey, Efrosyni Seitaridou
Abstract: An alternating electric field is applied at an ice interface to generate a resistive AC current having a frequency greater than 1000 Hz in interfacial ice. Typically, a first electrode and a second electrode proximate to the interface are separated by an interelectrode distance of about 50 ?m to 500 ?m. An AC power source provides a voltage of about 10 to 500 volts across the electrodes in order to create the alternating electric field. Interfacial ice converts capacitive AC current into resistive AC current, which generates Joule heat in the interfacial ice.
Abstract: The present invention provides a high-throughput screening assay to identify test agents as cholesterol inhibitors via mutant NCP1 mammalian cells. Also provided are cholesterol inhibiting agents identified in accordance with this assay and methods for using such agents to inhibit cholesterol accumulation in cells.
Abstract: The present invention provides cDNAs encoding deoxyribonuclease II? and isolated, purified deoxyribonuclease II? proteins. Antibodies against this protein and antisense agents targeted to a cDNA or corresponding mRNA encoding deoxyribonuclease II? are provided. In addition, methods of identifying and using modulators of deoxyribonuclease II? activity are described.
Type:
Grant
Filed:
March 1, 2004
Date of Patent:
May 23, 2006
Assignee:
Trustees of Dartmouth College
Inventors:
Alan Richard Eastman, Ronald J. Krieser
Abstract: A method modifies friction between an object and ice or snow. The method utilizes a heating element to apply a pulse of thermal energy to an interface between the object and the ice or snow; the first pulse is sufficient to melt an interfacial layer of ice at the interface. Water forming the interfacial layer refreezes to form a bond between the object and the ice or snow. The steps of the method may be repeated to form a second bond between the object and the ice or snow.