Abstract: The present invention provides a nucleotide mutation detection method wherein primers having a nucleotide sequence complementary to part of the nucleic acids to be detected and also having a nucleotide sequence, which is complementary to the nucleotide sequence just upstream from a nucleotide site corresponding to the mutation site of the nucleotide sequence formed at the 3? end, added to the 5? end, are produced so that so that the nucleotide site corresponding to the mutation site of the nucleic acids to be detected including the nucleotide mutation is located within the nucleotide sequence formed at the 3? end after elongation; a target is produced by subjecting the primers to an elongation reaction using polymerase or Klenow enzyme; the target is then denatured to a single strand, and is subjected to a hybridization reaction with a probe that has the nucleotide complementary to the mutation site of the nucleic acids present in the 3? end region and then to a ligation reaction, whereby it is enable to dete

Abstract: A detecting chip (2) comprising a body (5) and a frame (4) both detachable from each other. A large number of projecting pin electrodes (10) are arranged in a matrix inside the body (5). Oligonucleotide consisting of different gene sequences is fixed to the pin electrode (10). A common electrode is so disposed in a recess (8) in a frame (4) as to be out of contact with the pin electrodes (10). A DNA sample is placed in the recess (8). By applying a voltage between the common electrode and the pin electrodes (10), a current is detected to detect a hybridized two-strand DNA, thus analyzing a gene DNA.

Abstract: A gene detection system for detecting a target gene upon hybridization with a probe comprising a probe-immobilizing support on which a probe is immobilized, and heating and cooling means disposed in contact with another location different from the surface of the probe-immobilizing support on which the probe is immobilized, whereby genes can be rapidly detected with high sensitivity through hybridization.

Abstract: The present invention provides a gold-plated body, a method for surface treatment of a gold-plated body, a surface-treated product, and an immobilization method which make it possible to immobilize a large number of sulfur-containing molecules. With the surface treatment method in accordance with the present invention, the surface of a gold-plated body is subjected to an annealing treatment at a temperature of 350 to 790° C. so that a large number of sulfur-containing molecules can be immobilized thereon. In particular, the treatment is conducted so as to obtain a structure in which surface gold crystals have no less than 30% planes with (1, 1, 1) orientation. The present invention also provides a method for the manufacture of a gold-plated body that allows a large number of sulfur-containing molecules to be immobilized on the surface thereof, by which surface gold crystals are formed from a starting material comprising a crystal growth enhancer.

Abstract: The present invention provides a gold-plated body, a method for surface treatment of a gold-plated body, a surface-treated product, and an immobilization method which make it possible to immobilize a large number of sulfur-containing molecules. With the surface treatment method in accordance with the present invention, the surface of a gold-plated body is subjected to an annealing treatment at a temperature of 350 to 790° C. so that a large number of sulfur-containing molecules can be immobilized thereon. In particular, the treatment is conducted so as to obtain a structure in which surface gold crystals have no less than 30% planes with (1, 1, 1) orientation. The present invention also provides a method for the manufacture of a gold-plated body that allows a large number of sulfur-containing molecules to be immobilized on the surface thereof, by which surface gold crystals are formed from a starting material comprising a crystal growth enhancer.

Abstract: Space part S within a detecting chip 2 for single base substitution SNP and point mutation of genes where a plurality of gold electrodes 8 are formed in the base 7 of closed space part S, oligonucleotides 10 with different gene sequences are fixed to the gold electrodes 8, a common electrode 16 arranged not to contact the gold electrodes 8, are filled with DNA samples, voltage is applied between the common electrode 16 and the gold electrode 8, and current is measured to allow the double-stranded DNA to be detected and analyzed. It becomes possible to detect and analyze a large number of single base substitution SNP and point mutation for a plurality of DNA samples.

Abstract: A detection chip capable of detecting large amounts of genes with high sensitivity is provided. The gene detection chip comprises a plurality of pins 12 as measurement electrodes, a common electrode 22 as a counter electrode therefor, and a tabular member 14 with a plurality of pin holes 15 for accommodating the pins. An arrangement may be adopted in which the diameters of the pin holes 15 taper off in the direction in which the pins are inserted, and the pins are held in place in the narrowest sections of the pin holes 15.

Abstract: The present invention provides a blood testing method capable of accurately and easily performing quantitative analysis of the progression of tumors and the therapeutic reactivity, without placing any heavy burden on a patient, by detecting a mutation factor derived from tumor cells and a normal factor with no mutation in a sample containing human plasma with the mutation factor, and by calculating a relative ratio between the mutation factor amount and the normal factor amount.