Patents Assigned to TwistDx, Inc.
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Publication number: 20110065106Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.Type: ApplicationFiled: April 30, 2010Publication date: March 17, 2011Applicant: TwistDx, Inc.Inventors: Niall A. Armes, Derek L. Stemple
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Publication number: 20110053154Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.Type: ApplicationFiled: June 21, 2010Publication date: March 3, 2011Applicant: TwistDx, Inc.Inventors: Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
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Patent number: 7763427Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.Type: GrantFiled: May 7, 2008Date of Patent: July 27, 2010Assignee: TwistDx, Inc.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Patent number: 7759061Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.Type: GrantFiled: June 2, 2005Date of Patent: July 20, 2010Assignee: TwistDx, Inc.Inventors: Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
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Patent number: 7666598Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carryover contamination, and methods to employ sequence-specific third ‘specificity’ probes.Type: GrantFiled: April 11, 2005Date of Patent: February 23, 2010Assignee: TwistDx, Inc.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Publication number: 20090325165Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.Type: ApplicationFiled: January 30, 2009Publication date: December 31, 2009Applicant: TwistDx, Inc.Inventors: Niall A. Armes, Derek L. Stemple
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Patent number: 7485428Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.Type: GrantFiled: August 13, 2007Date of Patent: February 3, 2009Assignee: TwistDx, Inc.Inventors: Niall A. Armes, Derek L. Stemple