Patents Assigned to Two Blades Foundation
  • Publication number: 20240218356
    Abstract: Methods are provided for preparing a library of candidate plant disease resistance (R) genes against a plant pathogen of interest. The methods involve selecting from each of one or more plants of interest a subpopulation of highly expressed nucleotide-binding leucine rich repeat genes (NLRs) from among the population of NLRs that are constitutively expressed in an organ or other part of the one or more plants to produce a library of candidate R genes. Further provided are related methods for identifying R genes against a plant pathogen of interest using a library of candidate R genes and compositions comprising the identified R genes.
    Type: Application
    Filed: May 11, 2022
    Publication date: July 4, 2024
    Applicant: Two Blades Foundation
    Inventors: Matthew James Moscou, Hendrikus Pieter Van Esse
  • Patent number: 11697820
    Abstract: Compositions and methods and for enhancing the resistance of plants to a plant disease caused by a Phytophthora species are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a plant to a plant disease caused by a Phytophthora species comprise introducing a nucleic acid molecule encoding an R gene product into a plant cell. Additionally provided are methods for using the plants in agriculture to limit plant disease.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: July 11, 2023
    Assignee: Two Blades Foundation
    Inventors: Kamil Witek, Jonathan D. G. Jones
  • Patent number: 11510377
    Abstract: Disclosed herein are compostions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume plant.
    Type: Grant
    Filed: October 27, 2020
    Date of Patent: November 29, 2022
    Assignees: Two Blades Foundation, E. I. Du Pont De Nemours aand Company, Universidade Federal De Vicosa
    Inventors: Greg Rairdan, Karen Broglie, Gilda Rauscher, Hendrikus Pieter van Esse, Jonathan D. G. Jones, Cintia Goulart Kawashima, Sergio Herminio Brommonschenkel
  • Publication number: 20220251595
    Abstract: Compositions and methods for enhancing the disease resistance of plants are provided. The invention provides compositions comprising engineered, pattern recognition receptors which comprise one or more domains derived from the receptor-like protein AtRLP23, particularly a leucine-rich repeat domain, and one or more other domains including, for example, a kinase domain from a receptor-like kinase. The compositions further comprise nucleic acid molecules encoding the engineered proteins and plants, plant cells, and other host cells comprising such nucleic acid molecules and/or the engineered proteins. The invention additionally provides methods for making and using the engineered proteins and nucleic acid molecules encoding the engineered proteins.
    Type: Application
    Filed: June 26, 2020
    Publication date: August 11, 2022
    Applicant: Two Blades Foundation
    Inventors: Freddy France Guy Boutrot, Carolina Grandellis, Kamil Witek, Cyril B. Zipfel
  • Patent number: 11041166
    Abstract: Compositions and methods and for enhancing the resistance of plants to a plant disease caused by a Phytophthora species are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a plant to a plant disease caused by a Phytophthora species comprise introducing a nucleic acid molecule encoding an R gene product into a plant cell. Additionally provided are methods for using the plants in agriculture to limit plant disease.
    Type: Grant
    Filed: December 15, 2017
    Date of Patent: June 22, 2021
    Assignee: Two Blades Foundation
    Inventors: Kamil Witek, Hari S. Karki, Florian Gunter Jupe, Jonathan D. G. Jones
  • Patent number: 11041165
    Abstract: The present invention relates to the identification of the xcv-1 gene, which is responsible for a recessive resistance to Xanthomonas euvesicatoria, by genetic mapping-based cloning from Capsicum annuum. In addition, the invention relates to methods for generating plants resistant to an abiotic or biotic factor, in particular to Xanthomonas euvesicatoria, and the plants themselves, in particular tomato plants.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: June 22, 2021
    Assignee: Two Blades Foundation
    Inventors: György Botond Kiss, Zoltán Szabó, Carmen E Iliescu, Márta Balogh
  • Publication number: 20200362367
    Abstract: Compositions and methods and for enhancing the resistance of wheat plants to wheat stem rust caused by Puccinia graminis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a wheat plant to wheat stem mst comprise introducing a nucleic acid molecule encoding an R gene product into a wheat plant cell. Additionally provided are methods for using the wheat plants in agriculture to limit wheat stem rust.
    Type: Application
    Filed: January 14, 2019
    Publication date: November 19, 2020
    Applicant: Two Blades Foundation
    Inventors: Burkhard Steuernagel, Sanu Arora, Brande Wulff
  • Patent number: 9816103
    Abstract: Methods are provided for enhancing the resistance of plants to bacterial pathogens. The methods involve transforming a plant with a polynucleotide molecule comprising a plant promoter operably linked to a nucleotide sequence that encodes a plant receptor that binds specifically with bacterial elongation factor-Tu. Further provided are expression cassettes, transformed plants, seeds, and plant cells that are produced by such methods.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: November 14, 2017
    Assignee: Two Blades Foundation
    Inventors: Cyril B. Zipfel, Jonathan D. G. Jones
  • Patent number: 9222103
    Abstract: Methods are provided for enhancing the resistance of plants to bacterial pathogens. The methods involve transforming a plant with a polynucleotide molecule comprising a plant promoter operably linked to a nucleotide sequence that encodes a plant receptor that binds specifically with bacterial elongation factor-Tu. Further provided are expression cassettes, transformed plants, seeds, and plant cells that are produced by such methods.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: December 29, 2015
    Assignee: Two Blades Foundation
    Inventors: Cyril B. Zipfel, Jonathan D. G. Jones
  • Patent number: 9133467
    Abstract: Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided. Additionally, provided are R genes and other nucleic acid molecules comprising the pathogen-inducible promoters and that are made by such methods as well as plants, plant parts, plant cells, seeds, and non-human host cells comprising the R genes and other nucleic acid molecules.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: September 15, 2015
    Assignee: Two Blades Foundation
    Inventors: Thomas Lahaye, Patrick Römer, Sebastian Schornack, Jens Boch, Ulla Bonas
  • Publication number: 20130219555
    Abstract: Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided.
    Type: Application
    Filed: November 10, 2009
    Publication date: August 22, 2013
    Applicant: Two Blades Foundation
    Inventors: Thomas Lahaye, Patrick Römer, Sebastian Schornack, Jens Boch, Ulla Bonas
  • Patent number: 8198510
    Abstract: Isolated nucleic acid molecules that confer resistance to the plant pathogen Xanthomonas campestris are provided. These molecules may be introduced into plants that are otherwise susceptible to infection by this bacterium in order to enhance the resistance of the plant to this plant pathogen. Additionally provided are isolated polypeptides and isolated nucleic acid molecules comprising plant promoters. Methods of using the nucleic acid molecules to increase the resistance of plants to pathogens and to express genes of interest in plants are provided.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: June 12, 2012
    Assignee: Two Blades Foundation
    Inventors: Thomas Lahaye, Ulla Bonas, Patrick Römer
  • Publication number: 20100132069
    Abstract: Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided.
    Type: Application
    Filed: November 10, 2009
    Publication date: May 27, 2010
    Applicant: Two Blades Foundation
    Inventors: Thomas Lahaye, Patrick Rõmer, Sebastian Schornack, Jens Boch, Ulla Bonas
  • Publication number: 20100122376
    Abstract: Methods are provided for enhancing the resistance of plants to bacterial pathogens. The methods involve transforming a plant with a polynucleotide molecule comprising a plant promoter operably linked to a nucleotide sequence that encodes a plant receptor that binds specifically with bacterial elongation factor-Tu. Further provided are expression cassettes, transformed plants, seeds, and plant cells that are produced by such methods.
    Type: Application
    Filed: November 3, 2009
    Publication date: May 13, 2010
    Applicant: Two Blades Foundation
    Inventors: Cyril B. Zipfel, Jonathan D. G. Jones
  • Publication number: 20090133158
    Abstract: Isolated nucleic acid molecules that confer resistance to the plant pathogen Xanthomonas campestris are provided. These molecules may be introduced into plants that are otherwise susceptible to infection by this bacterium in order to enhance the resistance of the plant to this plant pathogen. Additionally provided are isolated polypeptides and isolated nucleic acid molecules comprising plant promoters. Methods of using the nucleic acid molecules to increase the resistance of plants to pathogens and to express genes of interest in plants are provided.
    Type: Application
    Filed: September 26, 2008
    Publication date: May 21, 2009
    Applicant: Two Blades Foundation
    Inventors: Thomas Lahaye, Ulla Bonas