Abstract: Methods and products for the identification and detection of new pancreatic cancer biomarkers based on proteins in biofluids, such as plasma and serum extracellular vesicles.

Abstract: A method is disclosed to capture and purify extracellular vesicles from biofluids via lipid affinity-based capture. The EVTRAP (Extracellular Vesicles Total Recovery And Purification) method enables capture of EVs onto modified beads. The EVTRAP method results in fast and reproducible capture and isolation of EVs with greater than 90% recovery yields.

Abstract: Methods and products for the identification and detection of new bladder cancer biomarkers based on proteins and protein phosphorylation in urinary extracellular vesicles.

Abstract: A method to isolate proteins from biofluids, such as plasma or uterine lavage, for biomarker discovery or for clinical detection. One or more biofluid samples can be provided wherein the one or more biofluids are selected from plasma or uterine lavage samples. Extracellular vesicles (EV) can be selectively and reversibly binded from the biofluid sample to capture and enrich the EVs of the biofluid sample using Extracellular Vesicles total recovery and purification (EVtrap) or other suitable method. At least one compound in the biofluid samples can be detected. The least one compound can consists of plasma or uterine lavage EV proteins and any combination thereof enriched from the plasma or uterine lavage resulting in a greater than 95% recovery yield, greater than 99% purity and with less than 5% coefficient of variation of the at least one EV compound.

Abstract: A method is disclosed to capture and purify extracellular vesicles from biofluids via lipid affinity-based capture. The EVTRAP (Extracellular Vesicles Total Recovery And Purification) method enables capture of EVs onto modified beads. The EVTRAP method results in fast and reproducible capture and isolation of EVs with greater than 90% recovery yields.