Abstract: The aim of the invention is to create a fundamentally new method of the substrate production, which would include the following: the processing of the parent materials, containing lignocellulose (for example, straw), by applying the methods of the primary processing (for example, steam explosion) in order to decompose the said parent material into the lower-level components; possible removal of the carbohydrates of the group C5 from the processed mass of lignocellulose; extrusion, pasteurization and sterilization of the initial substrate; adding of the components to the wetted initial substrate, its enrichment of materials necessary the growth of the mycelium and mushrooms; undersowing of the mycelium and its stirring in the volume of the prepared substrate.