Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a lest chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.
Type:
Application
Filed:
May 24, 2013
Publication date:
December 19, 2013
Applicants:
UCSD TECH TRANSFER OFFICE, THE JOHNS HOPKINS UNIVERSITY
Inventors:
Andre Levchenko, Alexander Groisman, Saurabh Paliwal
Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.
Type:
Grant
Filed:
October 11, 2006
Date of Patent:
May 28, 2013
Assignees:
The Johns Hopkins University, UCSD Tech Transfer Office
Inventors:
Andre Levchenko, Alexander Groisman, Saurabh Paliwal