Abstract: In part, the disclosure relates to an imaging system that includes a control system; image sensors in electrical communication with the control system, the one or more image sensors support data collection relative to a first pixel subwindow; and an optical assembly, the optical assembly oriented to receive light from the target and direct the light to the image sensor; wherein the optical assembly has an imaging focal volume within the imaging environment that spans a range of focus. The timing system is in electrical communication with the one or more image sensors, an illumination system and a translation assembly. A translation assembly may move target in imaging environment. The timing systems triggers illumination system to illuminate the target and the one or more sensors to image the target when image sensor pixel values in the first pixel subwindow align with at least a portion of the target.