Abstract: The invention concerns a diagnostic kit for simultaneous assay of antibiotics of different classes. The kit contains a single reaction mixture containing at least one first labelled receptor, specific of recognition of ?-lactams, a second labelled receptor, specifically and competitively identifying a tetracycline and a biotinylated nucleic acid fragment, an antisulfamide antibody and a labelled sulfamide analogue; and a recovery system in the form of a solid support comprising a nitrocellulose membrane whereon are fixed in three separate testing zones, respectively an antibiotic with ?-lactam nucleus, an avidin and an antibody capable of specifically identifying the antisulfamide antibody, so that the labelling intensity detected on the recovery system at the three testing zones results independently from a competitive recognition of each antibiotic by its labelled receptor.
Abstract: The invention concerns a kit for detecting and/or quantifying a ligant (C), present in an analysis sample, by a receptor (A), characterized in that it comprises the following reagents: a single-stranded or double-stranded nucleotide sequence (B), and said receptor (A) consisting of a monomeric or multimeric proteinic entity including at least a first recognition site specific for said ligand (C) and a second recognition site specific for said nucleotide sequence (B); said ligand (C) being capable of positively or negatively modulating fixing of the receptor (A) by its specific recognition site on the nucleotide sequence (B).
Abstract: The invention concerns a kit for detecting and/or quantifying a ligant (C), present in an analysis sample, by a receptor (A), characterized in that it comprises the following reagents: a single-stranded or double-stranded nucleotide sequence (B), and said receptor (A) consisting of a monomeric or multimeric proteinic entity including at least a first recognition site specific for said ligand (C) and a second recognition site specific for said nucleotide sequence (B); said ligand (C) being capable of positively or negatively modulating fixing of the receptor (A) by its specific recognition site on the nucleotide sequence (B).