Abstract: The present invention provides non-invasive diagnostic methods and kits for determining and/or monitoring oral health and glycemic control in subjects with Type 1 diabetes.
Type:
Grant
Filed:
April 21, 2015
Date of Patent:
September 5, 2017
Assignee:
UNIVERISTY OF SOUTH FLORIDA
Inventors:
Amy Christine Alman, Brant Roger Burkhardt
Abstract: The present invention provides materials and methods for detecting, quantifying, and/or profiling microRNAs. Advantageously, the present invention is sensitive, specific, convenient, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of miRNAs, a universal reverse primer for PCR amplification reaction, and a universal probe. In another embodiment, the present invention provides assays that allow the detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction and a single qPCR reaction.