Abstract: Xylose isomerase (XI) muteins useful in the conversion of glucose to fructose or xylose to xylulose are obtained in usable amounts by protein structural and recombinant DNA methods, including x-ray crystallography, cloning, computer graphic modeling and site-directed mutagenesis and expression of the bacterial DNA sequences encoding native procaryotic xylose isomerase. These native sequences are altered to encode the xylose isomerase muteins having improved catalytic function and/or thermostability.
Type:
Grant
Filed:
August 11, 1987
Date of Patent:
August 20, 1991
Assignees:
Cetus Corporation, Univerity of Oregon
Inventors:
Robert J. Drummond, Will Bloch, Brian W. Matthews, Pamela L. Toy