Abstract: The present disclosure provides for, among other things, methods and systems for detecting, diagnosing, predicting, monitoring, screening for, staging, and/or providing survival prognosis for advanced adenoma and/or colorectal cancer using DNA methylation markers. For example, described herein are DNA methylation markers that enable categorization and prognosis evaluation of advanced adenoma (AA) and/or colorectal cancer (CRC) patients with high accuracy from human biospecimens.

Abstract: Described herein are various systems, methods, and apparatus for identifying biomarkers for detection of a disease or condition such as advanced adenoma, colorectal cancer, or other cancers. In certain embodiments, the present invention relates to methods and/or systems for identifying one or more biomarkers related to methylation status in cell-free deoxyribonucleic acid (DNA) of a human subject and/or detecting (e.g., screening for) cancer based on one or more known biomarkers (e.g., the identified biomarkers).

Abstract: The present disclosure provides, among other things, methods of screening for colorectal cancer, methods of screening for advanced adenoma, methods of screening for the presence of either colorectal cancer or advanced adenoma (or both), and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that includes analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR.