Abstract: An epi-fluorescent microscope is constructed with a quick changing set of filters. A wheel containing a plurality of dichroic beamsplitter mirrors mounted equidistance from a center axis of the wheel is mounted at a 45° angle within the lightpath and provides a beamsplitter function. Light from an excitation source is provided as monochromatic light, so that light from the excitation source is reflected by a selected one of the dichroic mirrors to the specimen, and Stokes shifted light from the specimen is transmitted through the dichroic mirror. By separately mounting the dichroic mirrors, the dichroic mirrors can be quickly moved and a quicker change of sensed fluorophores is achieved.

Abstract: An epi-fluorescent microscope is constructed with a quick changing set of filters. A wheel containing a plurality of dichroic beamsplitter mirrors mounted equidistance from a center axis of the wheel is mounted at a 45° angle within the lightpath and provides a beamsplitter function. Light from an excitation source is provided as monochromatic light, so that light from the excitation source is reflected by a selected one of the dichroic mirrors to the specimen, and Stokes shifted light from the specimen is transmitted through the dichroic mirror. By separately mounting the dichroic mirrors, the dichroic mirrors can be quickly moved and a quicker change of sensed fluorophores is achieved.

Abstract: The invention provides an automated microscope system, a computer program product in a computer readable medium and a method for acquiring images at substantially the maximum acquisition rate of a camera while and as devices external to the camera, which vary various parameters of the acquired images, operate asynchronously with the camera so as to allow the acquired images to be displayed as a sequence that shows continuous variation in the acquisition parameters.

Abstract: An epifluorescent microscope (11) is constructed with a quick changing set of filters (13,20,21). A wheel (13) containing a plurality of dichroic beamsplitter mirrors (6165) mounted equidistance from a center axis (66) of the wheel is mounted at a 45° angle within the lightpath and provides a beamsplitter function. Light from an excitation source (12) is provided as monochromatic light, so that light from the excitation source (12) is reflected by a selected one of the dichroic mirrors (6165) to the specimen (15), and Stokes shifted light from the specimen (15) is transmitted through the dichroic mirror (6165). By separately mounting the dichroic mirrors (6165), the dichroic mirrors can be quickly moved and a quicker change of sensed fluorophores is achieved. An additional advantage is that excitation (20) and emission (21) filters can be quickly changed independently of the dichroic mirror element (13).