Abstract: The invention provides a product comprising: a membrane-spanning protein; a lipid membrane formed from amphiphilic molecules and membrane-spanning protein molecules; and a substrate, wherein the membrane protein is directly coupled to the substrate. The invention also provides a method for producing such a product which i) comprises treating a substrate with a hydrophilic coating agent; ii) providing at least one membrane-spanning protein; iii) bringing the protein into contact with the treated substrate under conditions for the coupling of the protein directly to the treated substrate; and iv) adding amphiphilic molecules to the protein-coupled substrate to form a lipid membrane. The product is useful for biosensors, protein arrays and the like.

Abstract: The present invention relates to fusion proteins (fusion polypeptides), particularly for use in expression and/or purification systems. The present inventors have found that the TolAIII domain has remarkable properties which are of particular use as a fusion protein partner to achieve high levels of expression in a host cell. In one aspect of the invention, a TolAIII domain or a functional homologue, fragment, or derivative thereof is located towards the N-terminus of the fusion polypeptide and a non-TolA polypeptide is located towards the C-terminus of the fusion polypeptide.

Abstract: An EKG structure comprising geosynthetic material associated with at least one conducting element wherein the structure comprises at least one core element substantially enclosed by at least one sheath, one or both comprising the geosynthetic material; the use thereof as an electrode, a method for treating substrate with the EKG structure, the treated substance obtained thereby.

Abstract: A fuel cell (1) having an electrode comprising an electrocatalyst (32) on a support, wherein the support is a mesh (30) of conductive material such as a metal, metal alloy and metal composite (e.g. titanium or titanium alloy), is disclosed, as well as a method of operating such a fuel cell by contacting a fuel and an oxidant on said electrode. The electrolyte of the fuel cell may be an ion exchange membrane.

Abstract: A resonant sensor for determining structural property changes, in particular for detecting the presence of chemical or biological species, comprises a structure (2) mounted to be capable of resonating and having a cyclically symmetrical configuration with two independent degenerative modes of vibration of a common natural frequency (f), and means (24,26,28,30,32,34,36,38) for exciting the structure (2) to resonate according to the two degenerative modes, regions (8,12,16,20) of the structure (2) being modified such that, on changes in the structural properties of the modified regions (8,12,16,20), for example by the addition or subtraction of mass, the natural frequencies (f1, f2) of the two modes of vibration become different, the difference in frequencies (?f) being proportional to the change in structural properties.

Abstract: An EKG structure comprising geosynthetic material associated with at least one conducting element wherein the structure comprises at least one core element substantially enclosed by at least one sheath, one or both comprising the geosynthetic material; the use thereof as an electrode, a method for treating substrate with the EKG structure, the treated substance obtained thereby

Abstract: An EKG structure comprising geosynthetic material associated with at least one conducting element wherein the structure comprises at least one core element substantially enclosed by at least one sheath, one or both comprising the geosynthetic material; the use thereof as an electrode, a method for treating substrate with the EKG structure, the treated substance obtained thereby

Abstract: A system for operating a command-input device for an automated production machine to provide commands to the machine includes a status signal generator; a computer and apparatus for operating the command-input device. Commands for various functions of the machine may be provided to the machine by operation of the command-input device. The status signals indicate functional states of the automated production machine; the computer responds to the status signals by generating control signals; and the apparatus for operating the command-input device of the automated production machine does so in response to the control signals. In an embodiment in which the command-input device is mechanically operated by using a keyboard or a touch screen, a robotic device is used to operate the command-input device in response to the control signals.

Abstract: Articles, such as components of an injection-molded multi-component product, are repositioned within a space, such as the space between mold parts, that is accessible for repositioning only during a series of periods, such as open-mold periods, that are separated by intervals of less accessibility for repositioning, such as closed-mold intervals by (a) moving a first article, such as a first component of a first multi-component product, from a first position within the space to outside of the space during a first such period; and (b) moving a second identical article, such as a first component of a second multi-component, from outside of the space to a second position within the space during the first such period. Both the movement of the first article and the movement of the second article are by means of a common vehicle, such as a common robot arm.

Abstract: A range of 3-oxybenzamide compounds and related quinazolinone compounds are disclosed which can act as potent inhibitors of the DNA repair enzyme poly(ADP-ribose) polymerase or PARP enzyme (EC 2.4.2.30), and which thereby can provide useful therapeutic compounds for use in conjunction with DNA-damaging cytotoxic drugs or radiotherapy to potentiate the effects of the latter. The coumpounds disclosed include 3-benzyloxybenzamides, 3-oxybenzamides in which a chain of 5 or more methylene groups terminate in a halogen atom or in a purin-9-yl moiety, certain benzoxazole-4-carboxamide compounds and certain quinazolinone compounds. In formula X and Y together may form a bride —X—Y— that represents the grouping (a), (b) or (c) wherein R5 is H, alkyl, aryl or aralkyl.

Abstract: Benzimidazole-4-carboxamide compounds (I) which can act as potent inhibitors of the DNA repair enzyme poly(ADP-ribose) polymerase or PARP enzyme (EC 2.4.2.30), and which thereby can provide useful therapeutic compounds for use in conjunction with DNA-damaging cytotoxic drugs or radiotherapy to potentiate the effects of the latter. In formula (I), R and R′ may each be selected independently from hydrogen, alkyl, hydroxyalkyl (e.g. CH2CH2OH), acyl (e.g. acetyl or benzoyl) or an optionally substituted aryl (e.g. phenyl) or aralkyl (e.g. benzyl or carboxybenzyl) group. R is generally a substituted phenyl group in the most preferred compounds. The compounds may also be used in the form of pharmaceutically acceptable salts or pro-drugs.

Abstract: A range of dipyridamole analogues useful for inhibiting transport of nucleosides or purines across cell membranes, thereby to potentiate the activity of various cytotoxic antitumor drugs, is disclosed.

Abstract: Various steps in the operation of an insulated runner injection molding system are automated by computer generated responses to various sensing steps or other steps to thereby reduce the performance requirements of the system operator. In automatic response to a manual operation incident to closure of a machine gate after an end-of sequence signal, a computer causes mold-cavity-encasing mold parts to be engaged. In automatic response to sensing such engagement of the mold-cavity-encasing mold parts, the computer causes the molding system to be adjusted to enable disengagement of insulated-runner-channel-encasing mold parts. In automatic response to sensing such adjustment of the molding system, the computer causes channel-encasing mold parts to be disengaged, whereupon a sprue is automatically removed therefrom in response to relative movement of the channel-encasing mold parts.

Abstract: Various steps in the operation of an insulated runner injection molding system are automated by computer generated responses to various sensing steps or other steps to thereby reduce the performance requirements of the system operator. In automatic response to a manual operation incident to closure of a machine gate subsequent to removal of a sprue from channel-encasing mold parts, a computer causes the molding system to be adjusted to enable injection of molten plastic into an insulated runner channel to form the insulated runner and through the insulated runner into mold-cavities. In automatic response to sensing such readjustment of the molding system, the computer causes a predetermined quantity of molten plastic to be metered for injection to form the insulated runner. In automatic response to sensing such metering the computer causes the metered quantity to be injected into the channel.

Abstract: Phosphate derivatives are disclosed of quinazolinone compounds having structural formula (I) or a pharmaceutically acceptable salt thereof, wherein X' represent hydroxyl, alkyl, alkoxy, or O--Z where Z is a phosphate or phosphate derivative; Y' represents hydrogen, alkyl or an optionally substituted aryl group or optionally substituted aralkyl group; and R' is hydrogen, alkyl, or CH.sub.2 --O--Z where Z is again a phosphate or phosphate derivative; subject to the proviso that if neither X' nor R' contains Z, Y' is an aryl or aralkyl group having an O--Z substituent therein with Z once again being a phosphate or phosphate derivative as hereinabove defined. These compounds are useful as prodrugs for providing active PARP inhibiting substances for medical use in conjunction with a cytotoxic drug or radiotherapy in order to increase the effectiveness of the latter, especially in connection with antitumor treatment.

Abstract: A carrier-member of a test species, or a component separated from a culture thereof, that may cause a desirable change in a behavior pattern of at least one member of an adjoiner species from an initial behavior pattern to a desired behavior pattern is identified by introducing a member of a test species that is a symbiont of the adjoiner species or a component separated from a culture of the symbiont test species to at least one member of the adjoiner species that has the initial behavior pattern; and subsequently collecting data that indicates whether or not the at least one member of the adjoiner species has acquired the desired behavior pattern to thereby make said identification.

Abstract: Benzimidazole-4-carboxamide compounds (I) which can act as potent inhibitors of the DNA repair enzyme poly(ADP-ribose) polymerase or PARP enzyme (EC 2.4.2.30), and which thereby can provide useful therapeutic compounds for use in conjunction with DNA-damaging cytotoxic drugs or radiotherapy to potentiate the effects of the latter. In formula (I), R and R' may each be selected independently from hydrogen, alkyl, hydroxyalkyl (e.g. CH.sub.2 CH.sub.2 OH), acyl (e.g. acetyl or benzoyl) or an optionally substituted aryl (e.g. phenyl) or aralkyl (e.g. benzyl or carboxybenzyl) group. R is generally a substituted phenyl group in the most preferred compounds. The compounds may also be used in the form of pharmaceutically acceptable salts or pro-drugs.

Abstract: Articles, such as components of an injection-molded multi-component product, are repositioned within a space, such as the space between mold parts, that is accessible for repositioning only during a series of periods, such as open-mold periods, that are separated by intervals of less accessibility for repositioning, such as closed-mold intervals by (a) moving a first article, such as a first component of a first multi-component product, from a first position within the space to outside of the space during a first such period; and (b) moving a second identical article, such as a first component of a second multi-component, from outside of the space to a second position within the space during the first such period. Both the movement of the first article and the movement of the second article are by means of a common vehicle, such as a common robot arm.

Abstract: A component of a member of a test species that deleteriously affects a target species that is a parasite of an adjoiner species is identified by a method including the steps of (a) selecting the test species from among test species that are attached or internal to a member of the adjoiner species whom was exposed to the target species but did not react adversely thereto as did other exposed members of the adjoiner species; (b) separating at least one member of the selected test species into a plurality of components; (c) exposing at least some of the separated components of the member(s) of the test species separately to members of the target species; and (d) examining such exposures to determine for such identification whether members of the target species have been deleteriously affected by such exposures. The test species may be a symbiont or a traditional food source of the adjoiner species.

Abstract: A bilayer of polymer film arranged in a manner to create respective flow paths for a first and second gaseous or liquid fluid, wherein the ratio of the surface area of polymer film adapted to contact and thereby isolate both fluids to the total matrix volume is in excess of 700 m.sup.2 /m.sup.3, processes for the preparation thereof, heat exchangers comprising such bilayers and uses thereof.