Abstract: The disclosed subject matter relates to a method for determining a three-dimensional particle distribution in a medium, comprising: emitting a coherent light beam to irradiate the sample; recording an interference image of the scattered light beam and a second part of the light beam that has not been scattered; computing, from the interference image, for each one of a plurality of virtual planes lying within the sample, a reconstructed image of the sample, generating for each reconstructed image, a presence image, wherein a value is assigned to each pixel of the presence images if the corresponding pixel of the reconstructed image has an intensity value exceeding a threshold value and if the corresponding pixel of the reconstructed image has a phase value with a predetermined sign.

Abstract: A ligand regulated protein-protein interaction system based on a lipocalin-fold molecule including: (a) a lipocalin-fold molecule; (b) a lipocalin-fold ligand with a low molecular weight of 1500 Da or below; and (c) a lipocalin-fold binding interaction partner; wherein the lipocalin-fold molecule can bind to the lipocalin-fold ligand; and wherein the lipocalin-fold molecule bound to the lipocalin-fold ligand binds to the lipocalin-fold binding interaction partner with an affinity which is at least 10-fold higher than the affinity of the lipocalin-fold molecule not bound to the lipocalin-fold ligand; and wherein the lipocalin-fold binding interaction partner is not a naturally occurring protein which has an affinity of <10 ?M to any naturally occurring lipocalin-fold molecule in the presence of any lipocalin-fold ligand.

Abstract: The present invention relates in general to the use of a kit or composition comprising at least one yeast and at least one bacterium, selected from the group consisting of lactobacillales, rhizobiales and bifidobacteriales, and/or comprising calcium for improving plant health, for improving plant resistance to plant pathogens, for preventing or reducing mycotoxin contamination of plant material, for improving plant resistance to a plant disease, for preventing perithecia formation of a plant pathogen on plant debris, for plant protection and/or as plant stimulant. The present invention also relates to corresponding compositions and kits and a method of applying such composition or kit to a living plant or plant debris.

Abstract: The present invention provides a method of detecting senescent cells or diagnosing cellular senescence in a subject wherein the level of one or more selected miRNAs is quantified in a sample from said subject.

Abstract: A method for determining a focal length of a particle in a medium, wherein the method comprises: providing a sample; emitting a coherent light beam to irradiate the sample, wherein a first part of the light beam is scattered by the particle; recording an interference image; computing for a set of positions an electric field from the interference image; generating a representation comprising, for each of said positions, an intensity value of the coherent light beam calculated from the computed electric field; finding two positions, a first of which lies in the sample, a second of which lies in the beam direction behind or in front of said first position; and determining the focal length from the two found positions.

Abstract: A method for determining a property of at least one particle (P) in a medium (M), comprising: emitting a coherent light beam (6) to irradiate a sample (5) of the medium (M); recording an interference image (10) of a first part of the light beam (6) scattered by the at least one particle (P) and a second part of the light beam (6) not scattered by particles (P); computing (S1), for positions (13) within the sample (5), an electric field (E1) of the first part from the interference image (10); generating (S2) a representation (16) comprising for each position (13), a phase value (?), determined from the computed electric field (E1) and from an estimated electric field (E2) of the second part, and an intensity value (I), determined from the intensity (I1) of the first part and from the phase value (?); and determining (S3), therefrom, said property.

Abstract: A method is provided for preparing a water-insoluble lignosulfonate polymer from a first solution containing lignosulfonate precursors, which involves a) separating the lignosulfonate precursors from the first solution and providing a second solution with at least 5 wt % of lignosulfonate precursors obtained from the first solution, wherein the separating comprises filtering out components with a predefined particle diameter and separating low-molecular weight components including salts and low-molecular organic compounds, b) adding a radical-forming enzyme and gas to the second solution, wherein the gas comprises oxygen, and c) polymerizing the lignosulfonate precursors of the resultant solution of b). Other related aspects are described and claimed.

Abstract: The disclosed subject matter relates to a method for determining a three-dimensional particle distribution in a medium, comprising: emitting a coherent light beam to irradiate the sample; recording an interference image of the scattered light beam and a second part of the light beam that has not been scattered; computing, from the interference image, for each one of a plurality of virtual planes lying within the sample, a reconstructed image of the sample, generating for each reconstructed image, a presence image, wherein a value is assigned to each pixel of the presence images if the corresponding pixel of the reconstructed image has an intensity value exceeding a threshold value and if the corresponding pixel of the reconstructed image has a phase value with a predetermined sign.

Abstract: A method of preparing a vesicular particle having at least in part a lipid and/or polymeric membrane that is a barrier between the interior and exterior of the vesicular particle, wherein the membrane includes at least one inorganic core nanoparticle embedded in the membrane, the method includes the steps of i) providing a first dispersion with one or more inorganic core particles having a hydrophobic dispersant shell, in a solution of membrane forming lipids and/or polymers in a non-aqueous solvent; and ii) introducing the first dispersion into a non-solvent for the membrane forming lipids and/or polymers, wherein the volume of the non-solvent exceeds the volume of the first dispersion, thereby forming the vesicular particles; the produced particle preparations and their uses.

Abstract: The field of the invention relates to a method for the production of a recombinant protein in a plant-based system comprising the steps of providing a plant-based system comprising a modulation for a plant endogenous prolyl-4-hydroxylase gene, delivering a gene encoding the recombinant protein into the plant-based system, and cultivating the plant-based system for the expression of the gene encoding the recombinant protein. The field of the invention further relates to a recombinant protein, which has been produced in a plant-based system. A plant-based system and use of the recombinant protein are also provided.

Abstract: Panels of mammalian cell samples, kits and methods of use thereof. The panels comprise samples or sets of samples of immortalized primary cells, obtained from different tissues and organs of healthy and/or diseased donors. The cell panels are useful in assays for testing effects of drug candidates, e.g. for activity and toxicity.

Abstract: The invention relates to methods for diagnosing bone disorders and/or cardiovascular disorders in a subject, by contacting a biological sample from the subject with (a) a nucleic acid molecule which hybridizes to miR-31 or its 5 or 3? isoforms or variants, or (b) an agent that binds to miR-31 or its 5? or 3? isoforms or variants; detecting and evaluating the hybridization signal of the nucleic acid molecule or agent with a polynucleotide or said miR-31 or its 5? or 3? isoforms or variants; and comparing the detected and evaluated hybridization or binding signal with that of a control sample, wherein a stronger hybridization signal or a stronger binding signal in the sample of the subject compared to that of the control sample is indicative for a risk of developing or having a bone disorder and/or cardiovascular disorder.

Abstract: The invention relates to methods for diagnosing bone disorders and/or cardiovascular disorders in a subject, by contacting a biological sample from the subject with (a) a nucleic acid molecule which hybridizes to miR-31 or its 5 or 3? isoforms or variants, or (b) an agent that binds to miR-31 or its 5? or 3? isoforms or variants; detecting and evaluating the hybridization signal of the nucleic acid molecule or agent with a polynucleotide or said miR-31 or its 5? or 3? isoforms or variants; and comparing the detected and evaluated hybridization or binding signal with that of a control sample, wherein a stronger hybridization signal or a stronger binding signal in the sample of the subject compared to that of the control sample is indicative for a risk of developing or having a bone disorder and/or cardiovascular disorder

Abstract: The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.

Abstract: The present invention relates to new galactosyltransferases, nucleic acids encoding them, as well as recombinant vectors, host cells, antibodies, uses and methods relating thereto.