Abstract: A method for the in vitro diagnosis of an invasive fungal infection by MALDI-TOF mass spectrometry. The method involves, providing a liquid biological sample from a mammal, said biological sample containing, in particular, proteins and/or lipids and/or salts and/or polysaccharides and/or oligosaccharides and/or monosaccharides capable of forming complexes with said proteins and/or lipids and/or salts; treating said sample with biological liquid so as to extract said polysaccharides and/or oligosaccharides and/or monosaccharides; determining, by MALDI-TOF mass spectrometry, whether or not there is present among said extracted polysaccharides, oligosaccharides and/or monosaccharides, at least one given compound of interest coming from said fungal microorganism and chosen from polysaccharides, oligosaccharides and monosaccharides; and deducing, if said given compound of interest is present in said sample, that said mammal is suffering from an invasive fungal infection.
Type:
Grant
Filed:
June 24, 2013
Date of Patent:
June 7, 2016
Assignees:
Centre Hospitalier Regional Universitaire De Lille, Universite De Droit Et Sante De Lille, Universite Sciences Technologies Lille, Centre National De La Recherche Scientifique
Inventors:
Daniel Poulain, Boualem Sendid, Yann Guerardel, Nadine Francois
Abstract: A method for preparing a biomaterial containing at least one bioactive molecule from a base biomaterial, comprising the following successive operations carried out on the base biomaterial: a) application of a solid mixture of: cyclodextrin(s) and/or cyclodextrin derivative(s) and/or cyclodextrin inclusion complex(es) and/or cyclodextrin derivative inclusion complex(es), at least one poly(carboxylic) acid, and optionally a catalyst; b) heating at a temperature between 100° C. and 200° C. for a period of 1 to 60 minutes; c) washing with water; d) drying, wherein at least one bioactive agent is incorporated in the biomaterial by impregnation of the biomaterial after the drying step in a concentrated solution of the bioactive agent.
Type:
Grant
Filed:
November 15, 2005
Date of Patent:
December 9, 2014
Assignees:
Universite des Sciences et Technologies de Lille SAIC, Universite de Droit et Sante
Inventors:
Bernard Martel, Nicolas Blanchemain, François Boschin, Stephan Haulon, Elisabeth Delcourt Debruyne, Michel Morcellet, Hartmut Friederich Hildebrand